Rat IgG2c Negative Control antibody

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Rat IgG2c Negative Control

Product Type: Negative/Isotype Control
Isotype: IgG2c
Specificity: Rat IgG2c Negative Control

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References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Rat IgG2c negative control antibody is suitable for use as a negative control on human and dog tissues where rat monoclonals of isotype IgG2c are used.

Target Species: Negative Control
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Carrier Free: Yes
Immunogen: Isolated C3H thymic stromal cells.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Fusion Partners: Spleen cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.56 myeloma cell line.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. *This antibody should be used at the same concentration as the test antibody.

Flow Cytometry: *It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.

References for Rat IgG2c Negative Control antibody

Karbyshev, M.S. et al. (2018) Development of Novel Monoclonal Antibodies for Evaluation of Transmembrane Prostate Androgen-Induced Protein 1 (TMEPAI) Expression Patterns in Gastric Cancer.
Pathol Oncol Res. 24 (2): 427-38.