Rat IgG2b Negative Control antibody

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Rat IgG2b Negative Control Antibody gallery image 1 — Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat IgG2b isotype control (MCA6006F). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat anti Human CD18 (MCA503F). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Rat IgG2b Negative Control Antibody gallery image 2 — Figure A. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat IgG2b isotype control (MCA6006PE). Figure B. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat anti Human CD28 (MCA709PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Rat IgG2b Negative Control Antibody gallery image 3 — Figure A. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control (MCA6006F). Figure B. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat anti Canine CD4 FITC (MCA1038F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG2b Negative Control:APC

Product Type: Negative/Isotype Control
Isotype: IgG2b
Specificity: Rat IgG2b Negative Control

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Rat IgG2b negative control, a rat monoclonal raised against KLH, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2b test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.

Target Species: Negative Control
Product Form: Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Reconstitution: Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Immunogen: KLH
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

APC 650 661
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
APC	650	661

Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.