Rat IgG2b Negative Control antibody

100% Secure


Rat IgG2b Negative Control:Alexa Fluor® 488

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:Alexa Fluor® 647

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:APC

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:Biotin

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:FITC

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:Low Endotoxin

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:Pacific Blue®

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:RPE

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:RPE-Alexa Fluor® 647

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:RPE-Alexa Fluor® 750

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Rat IgG2b Negative Control:Alexa Fluor® 700

Rat IgG2b isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes keyhole limpet hemocyanin (KLH).

Product Type
Negative/Isotype Control
Isotype
IgG2b
Isotype controls savings up to 65%
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6006A488 F 100 Tests/1ml
MCA6006A647 F 100 Tests/1ml
MCA6006APC F 100 Tests
MCA6006B F 0.1 mg
MCA6006F F 0.1 mg
MCA6006EL F 0.5 mg
MCA6006PB F 100 Tests/1ml
MCA6006GA F 0.1 mg
MCA6006 F 1 mg
MCA6006PE F 100 Tests
MCA6006P647 F 100 Test
MCA6006P750 F 100 Tests/1ml
MCA6006A700 F 100 Tests/1ml
Rat IgG2b negative control, a rat monoclonal raised against KLH, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2b test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.

Product Details

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE): Alexa Fluor® 647 - lyophilised
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE): Alexa Fluor® 750 - lyophilized
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 1 ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of reconstitution
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of reconstitution
Shelf Life
12 months from date of reconstitution
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Rat IgG2b Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul