HuCAL Fab-F-Spy2-H Negative Control antibody | AbD18705ad

HuCAL Fab-F-Spy2-H Negative Control Antibody

Product Type
Negative/Isotype Control
Clone
AbD18705ad
Isotype
HuCAL Fab monovalent
Specificity
HuCAL Fab-F-Spy2-H Negative Control

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TZA003
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HuCAL Fab-F-Spy2-H negative control, clone AbD18705ad is a recombinant Fab antibody fragment in the format Fab-F-Spy2-H with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It has been tested by ELISA for specificity to GFP, but has not been tested in other applications. It is suggested as a negative control reagent when using other HuCAL antibodies of the same Fab format.

It is recommended that this reagent is used at the same concentration as the test reagent.

Target Species
Negative Control
Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library. Expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% ProClin 950
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyCatcher2 TZC001 IY 1 mg loader
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Description SpyCatcher2
SpyCatcher2-CYS TZC001CYS IY 1 mg loader
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Description SpyCatcher2-CYS
BiSpyCatcher2 TZC002 IY 1 mg loader
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Description BiSpyCatcher2
BiSpyCatcher2-CYS TZC002CYS IY 1 mg loader
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Description BiSpyCatcher2-CYS
BiSpyCatcher2-CYS3 TZC002CYS3 IY 1 mg loader
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Description BiSpyCatcher2-CYS3
Human IgG1-FcSpyCatcher3 TZC009 IY 1 mg loader
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Description Human IgG1-FcSpyCatcher3
Mouse IgG2a-FcSpyCatcher3 TZC012 IY 0.5 mg loader
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Description Mouse IgG2a-FcSpyCatcher3
Rabbit IgG-FcSpyCatcher3 TZC013 IY 0.5 mg loader
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Description Rabbit IgG-FcSpyCatcher3
Human IgG2-FcSpyCatcher3 TZC016 IY 0.5 mg loader
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Description Human IgG2-FcSpyCatcher3
Human IgG3-FcSpyCatcher3 TZC017 IY 0.5 mg loader
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Description Human IgG3-FcSpyCatcher3
Human IgG4-FcSpyCatcher3 TZC018 IY 0.5 mg loader
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Description Human IgG4-FcSpyCatcher3
Human IgG4-Pro-FcSpyCatcher3 TZC019 IY 0.5 mg loader
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Description Human IgG4-Pro-FcSpyCatcher3
Human IgA-FcSpyCatcher3 TZC020 IY 0.5 mg loader
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Description Human IgA-FcSpyCatcher3
SpyCatcher3 TZC025 IY 1 mg loader
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Description SpyCatcher3
SpyCatcher3-CYS TZC025CYS IY 1 mg loader
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Description SpyCatcher3-CYS

Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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