SARS-CoV-2 Nucleoprotein
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SARS-CoV-2 Nucleoprotein
- Product Type
- Antigen
- Specificity
- SARS-CoV-2 Nucleoprotein
| Recombinant SARS-CoV-2 nucleoprotein is a purified preparation of the SARS CoV-2 nucleoprotein. The nucleoprotein (N) is the most abundant viral protein in SARS-CoV-infected cells (Chang et al. 2014). It is one of the four structural proteins essential for viral assembly, alongside the spike (S), envelope (E), and membrane (M) proteins (Bartlam et al. 2005). The nucleoprotein encloses the viral genome; during assembly of the virion, nucleoprotein binds to viral RNA and forms the helical nucleocapsid and appears to play an important role in enhancing viral transcription (Zhu et al. 2004). Mouse anti SARS-CoV Nucleoprotein MCA6372 is recommended for use as a capture antibody in ELISA with Mouse anti SARS-CoV Nucleoprotein MCA6373 as a detection antibody. |
- Target Species
- Viral
- Species Cross-Reactivity
-
Target Species Cross Reactivity Viral - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified recombinant protein - liquid
- Preparation
- Recombinant SARS-CoV-2 full-length nucleoprotein, expressed and purified from E. coli with a 6x His-tag.
- Source
- E.coli
- Buffer Solution
- 20mM Sodium Phosphate
25mM Potassium Carbonate pH10.0
150mM Sodium Chloride - Preservative Stabilisers
- None present
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.45 mg/ml
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Capture Antibody:
MCA6372
ELISA Matched Pair-Detection Antibody:
MCA6373
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- A0A6C0T6Z7
PIP054
160113
167927
167993
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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