SARS-CoV-2 Nucleoprotein

SARS-CoV-2 Nucleoprotein

Product Type
Antigen
Specificity
SARS-CoV-2 Nucleoprotein

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PIP054
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Recombinant SARS-CoV-2 nucleoprotein is a purified preparation of the SARS CoV-2 nucleoprotein.

The nucleoprotein (N) is the most abundant viral protein in SARS-CoV-infected cells (Chang et al. 2014). It is one of the four structural proteins essential for viral assembly, alongside the spike (S), envelope (E), and membrane (M) proteins (Bartlam et al. 2005). The nucleoprotein encloses the viral genome; during assembly of the virion, nucleoprotein binds to viral RNA and forms the helical nucleocapsid and appears to play an important role in enhancing viral transcription (Zhu et al. 2004).

Mouse anti SARS-CoV Nucleoprotein MCA6372 is recommended for use as a capture antibody in ELISA with Mouse anti SARS-CoV Nucleoprotein MCA6373 as a detection antibody.

Target Species
Viral
Species Cross-Reactivity
Target SpeciesCross Reactivity
Viral
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified recombinant protein - liquid
Preparation
Recombinant SARS-CoV-2 full-length nucleoprotein, expressed and purified from E. coli with a 6x His-tag.
Buffer Solution
20mM Sodium Phosphate
25mM Potassium Carbonate pH10.0
150mM Sodium Chloride
Preservative Stabilisers
None present
Approx. Protein Concentrations
Current, batch-specific concentration 1.88 mg/ml
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6372 ELISA Matched Pair-Detection Antibody: MCA6373
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.

Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt
A0A6C0T6Z7
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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