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Native Human IgM

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5275-5504 E 5 mg
Native Human IgM is purified from human plasma and demonstrated a single band in electrophoresis. Native Human IgM has been used as the IgM antigen in the development of a sensitive ELISA assay for the measurement of IgM in human samples.

Product Details

Target Species
Human
Product Form
Purified immunoglobulin from human plasma - lyophilised
Reconstitution
Reconstitute with 1ml 250mM Sodium Chloride. Further dilutions may then be made in sterile Phosphate Buffered Saline.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgM prepared by delipidation, salt fractionation and ion-exchange chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
None present.
Purity
>95% Single band in high resolution protein electrophoresis on Corning HRPE agarose gel. 0.5 µl of IgM solution at 50 mg/ml in isotonic saline.
Approx. Protein Concentrations
5.0 mg/ml after reconstitution

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01871 Related reagents
Entrez Gene
IGHM Related reagents
GO Terms
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005624 membrane fraction
Regulatory
For research purposes only

Applications of IgM

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.