IgG

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Native Human IgG

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5172-9017 E 25 mg
Native human IgG is a preparation of human of human IgG derived from a single patient with myeloma. This preparation of purified IgG yields a single band on gel electrophoresis.

Product Details

Target Species
Human
Product Form
Purified immunoglobulin from human serum - lyophilised
Reconstitution
Reconstitute with 5.0 ml PBS and mix well. Heating to 37oC may aid reconstitution.

Following reconstitution, storage at +4oC and the addition of 0.09% sodium azide is recommended.
Preparation
Purified IgG isolated from a single donor containing Bence Jones protein.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Purity
>95%

Storage Information

Storage
Prior to reconstitution store at +4oC or -20oC. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution storage at +4oC is recommended. This product should be stored undiluted.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P01860 Related reagents
P01857 Related reagents
P01859 Related reagents
P01861 Related reagents
P01834 Related reagents
P0CG04 Related reagents
Entrez Gene
IGKC Related reagents
IGHG1 Related reagents
IGHG2 Related reagents
IGHG3 Related reagents
IGHG4 Related reagents
IGLV2-14 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IgG

  1. Bustanji, Y. et al. (2016) Erythropoietin Biosimilar Products and Immunogenicity: A Pharmacovigilance Study.
    Int J Pharmacol Res 6: 200-5.