IgG

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5172-9017
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Native human IgG is a preparation of human of human IgG derived from a single patient with myeloma. This preparation of purified IgG yields a single band on gel electrophoresis.

Target Species
Human
Product Form
Purified immunoglobulin from human serum - lyophilized
Reconstitution
Reconstitute with 5.0 ml PBS and mix well. Heating to 37oC may aid reconstitution.

Following reconstitution, storage at +4oC and the addition of 0.09% sodium azide is recommended.
Preparation
Purified IgG isolated from a single donor containing Bence Jones protein.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Purity
>95%
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG

  1. Bustanji, Y. et al. (2016) Erythropoietin Biosimilar Products and Immunogenicity: A Pharmacovigilance Study.
    Int J Pharmacol Res 6: 200-5.

RRID
AB_906193
UniProt
P01860
P01857
P01859
P01861
P01834
P0CG04
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products