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Anti-Sarilumab Antibodies
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Bioanalytical Antibodies
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Antibodies for Bioanalysis and Drug Monitoring
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Our Anti-Biotherapeutic Antibodies
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Our Anti-Biotherapeutic Antibodies
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Antibodies for Bioanalysis and Drug Monitoring
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Antibodies for bioanalysis and drug monitoring of sarilumab
Develop highly selective and sensitive PK and ADA assays for the humanized antibody drug sarilumab (Kevzara) using our range of ready-made anti-idiotypic antibodies.
- Inhibitory antibodies specific to sarilumab
- Fully human surrogate positive control or calibrator
- Sequence defined, well characterized reagents with secure supply
- Recombinant production, stringent quality control for batch to batch consistency
- Non-animal-derived antibodies, reducing the use of animals in science
Table 1. Antibodies Specific to Sarilumab.
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
|---|---|---|---|---|---|---|
|
Sarilumab Inhibitory Type 1 |
HCA410 |
AbD49514ia |
hIgG1 |
<0.1 |
ADA assay |
|
|
HCA411 |
AbD49516ia |
hIgG1 |
0.9 |
ADA assay |
||
|
HCA412 |
AbD49520ia |
hIgG1 |
2 |
ADA assay |
||
|
TZA046 |
AbD5019ad |
Fab-F-Spy2-H |
0.02 |
PK bridging ELISA (capture) |
||
|
TZA047 |
AbD51309ad |
Fab-F-Spy2-H |
0.3 |
PK bridging ELISA (detection) |
||
|
TZA047P |
AbD51309pap |
Fab2-FH-X22-HRP |
0.3 |
PK bridging ELISA (detection) |
* Affinity measured in the monovalent Fab format
1 Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
2 Fab2-FH-X22-HRP= Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags and Fab-SpyTag coupled to HRP conjugated BiCatcher
Anti-Sarilumab Inhibitory Antibodies (Type 1)
Type 1 anti-sarilumab antibodies inhibit the binding of the drug sarilumab to its target, the human interleukin-6 receptor (IL-6R). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. The antibody in fully human IgG1 format is suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.
TrailBlazer Antibodies
TrailBlazer™ Antibodies, clones TZA046 (AbD5019ad) and TZA047 (AbD51309ad) have a SpyTag2 incorporated at the C-terminus of the antibody heavy chain. In a fast reaction that simply requires mixing at room temperature, these antibodies can form a covalent isopeptide bond to a SpyCatcher reagent, enabling site-directed conjugation or fast conversion to a bivalent Fab or a full-length Ig-like format within an hour.
ELISA Protocols to Get You Started
PK Bridging ELISA
Fig. 1. Sarilumab PK bridging ELISA using antibodies TZA046 and TZA047P.
Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Fab format (purple) labeled with HRP.
ADA Bridging ELISA
Fig. 2. Sarilumab ADA bridging ELISA using antibody HCA410, HCA411 and HCA412.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
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