Anti-Isatuximab Antibodies

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  • Anti-Isatuximab Antibodies
  • Anti-Isatuximab Inhibitory Antibodies (Type 1)
  • ELISA Protocols to Get You Started
  • Resources

Antibodies for bioanalysis and drug monitoring of isatuximab

Develop highly selective and sensitive PK and ADA assays for the humanized antibody drug isatuximab (Sarclisa®) using our range of ready-made anti-idiotypic antibodies.

  • Recombinant inhibitory antibodies specific to isatuximab
  • TrailBlazer™ Antibodies enabling site-directed conjugation and format switching
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch-to-batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Isatuximab.

Specificity
Binding Type		 Catalog Number Clone Format Affinity* KD, nM Assay Recommendation

Product Details

Isatuximab Inhibitory Type 1

HCA441

AbD64591ia

hIgG1

0.2

ADA assay

Order HCA441

HCA442

AbD64596ia

hIgG1

0.2

ADA assay

Order HCA442

HCA443

AbD64597ia

hIgG1

0.4

ADA assay

Order HCA443

TZA0124 AbD64599ad Fab-F-Spy2-H1 1.0

PK bridging ELISA (capture)

Order TZA0124
TZA0125 AbD64592ad Fab-F-Spy2-H1 1.0

PK bridging ELISA (detection)

Order TZA0125
TZA0125P AbD64592pap Fab2-FH-X22-HRP2 1.0

PK bridging ELISA (detection)

Order TZA0125P

* Affinity measured in the monovalent Fab format.
1 Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
2 Fab2-FH-X22-HRP = Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP-conjugated BiCatcher.

Anti-Isatuximab Inhibitory Antibodies (Type 1)

Type 1 anti-isatuximab antibodies inhibit the binding of the drug isatuximab to its target, CD38. They are ideal for the development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In a fully human IgG1 format, they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.

ELISA Protocols to Get You Started

PK Bridging ELISA

PK Bridging ELISA, Free Drug: TZA0124 (capture) and TZA0125P (detection)

Fig. 1. Isatuximab PK bridging ELISA using antibodies TZA0124 (capture) and TZA0125P (detection).

Fig. 1. Isatuximab PK bridging ELISA using antibodies TZA0124 (capture) and TZA0125P (detection).

Schematic image of PK bridging ELISA measuring free drug

Schematic image of PK bridging ELISA detecting free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Fab format (purple), labeled with HRP.

ADA Bridging ELISA

ADA Bridging ELISA: HCA441, HCA442 and HCA443

Fig. 2. Isatuximab ADA bridging ELISA using HCA441, HCA442 and HCA443.

Fig. 2. Isatuximab ADA bridging ELISA using HCA441, HCA442 and HCA443.

Immunogenicity Assay – Bridging Format

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Resources

Anti-idiotypic antibodies, their binding types and properties

Anti-biotherapeutic antibody quality control and characterization

Custom anti-idiotypic antibody generation service

Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.