Anti-Emicizumab Antibodies

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Antibodies for bioanalysis and drug monitoring of emicizumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for the humanized antibody bispecific drug emicizumab (Hemlibra) using our range of ready-made anti-idiotypic antibodies.

• Inhibitory antibodies specific to emicizumab
• Fully human surrogate positive control or calibrator
• Sequence defined, well characterized reagents with secure supply
• Recombinant production, stringent quality control for batch-to-batch consistency
• Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Emicizumab

* Affinity measured in the monovalent Fab format.
¹ Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
² Fab2-FH-X22-HRP = Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP-conjugated BiCatcher.

Anti-Emicizumab Inhibitory Antibodies (Type 1)

Type 1 anti-emicizumab antibodies inhibit the binding of the bispecific drug emicizumab to its targets. TZA066 (AbD50384ad, Fab-F-Spy2-H format) is a paratope-specific, anti-idiotypic (Type 1) antibody that specifically recognizes the humanized monoclonal antibody emicizumab, namely the Factor IXa binding part of emicizumab. TZA067 and TZA067P are paratope-specific, anti-idiotypic antibodies (Type 1) that specifically recognize the humanized monoclonal antibody emicizumab, namely the Factor X binding part of emicizumab. Type 1 antibodies do not recognize free human Factor IXa or Factor X, nor emicizumab in complex with either human Factor IXa or Factor X. They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.

ELISA Protocols to Get You Started

PK Bridging ELISA

Fig. 1. Emicizumab PK bridging ELISA using antibodies TZA066 (capture) and TZA067P (detection).

Schematic image of PK bridging ELISA detecting free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Fab format (purple), labeled with HRP.

ADA Bridging ELISA

Fig. 2. Emicizumab ADA bridging ELISA using antibodies HCA436 or HCA437.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Resources

Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.