Anti-Belimumab Antibodies
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Bioanalytical Antibodies
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Antibodies for Bioanalysis and Drug Monitoring
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Our Anti-Biotherapeutic Antibodies
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Antibodies for Bioanalysis and Drug Monitoring
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Antibodies for bioanalysis and drug monitoring of belimumab and biosimilars
Develop highly selective and sensitive PK and ADA assays for the humanized antibody drug belimumab (Benlysta®) using our range of ready-made anti-idiotypic antibodies.
- Highly specific to belimumab and the belimumab/BAFF complex
- Inhibitory and non-inhibitory antibodies specific to belimumab
- Fully human surrogate positive control or calibrator
- Sequence defined, well characterized reagents with secure supply
- Recombinant production, stringent quality control for batch-to-batch consistency
- Non-animal-derived antibodies, reducing the use of animals in science
Table 1. Antibodies Specific to Belimumab.
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
|---|---|---|---|---|---|---|
|
Belimumab |
HCA429 |
AbD60138ia |
Human IgG1 |
16 |
ADA assay |
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HCA430 |
AbD60136ia |
Human IgG1 |
3 |
ADA assay |
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HCA431 |
AbD60132ia |
Human IgG1 |
0.2 |
ADA assay |
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|
TZA0100 |
AbD60131ad |
Fab-F-Spy2-H1 |
0.3 |
PK bridging ELISA |
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|
TZA0100P |
AbD60131pap |
Fab2-FH-X22-HRP2 |
0.3 |
PK bridging ELISA |
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|
Belimumab & Belimumab/BAFF Non-Inhibitory Type 2 |
TZA0101 |
AbD59900ad |
Fab-F-Spy2-H1 |
2 |
PK bridging ELISA |
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TZA0102 TZA0102P |
AbD59897ad AbD59897pap |
Fab-F-Spy2-H1 Fab2-FH-X22-HRP2 |
85 85 |
PK bridging ELISA |
* Affinity measured in the monovalent Fab format.
1 Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
2 Fab2-FH-X22-HRP = Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP-conjugated BiCatcher.
Anti-Belimumab Inhibitory Antibodies (Type 1)
Type 1 anti-belimumab antibodies inhibit the binding of the drug belimumab to its target, B-cell activating factor (BAFF). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format, they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.
Anti-Belimumab Non-Inhibitory Antibodies (Type 2)
The Type 2 anti-belimumab antibody binds to the belimumab idiotope but does not inhibit the binding of belimumab to BAFF. They are ideal for development of a PK bridging ELISA to measure total belimumab (free and BAFF-bound belimumab).
ELISA Protocols to Get You Started
PK Bridging ELISA
Fig. 1. Belimumab PK bridging ELISA using antibodies TZA0100 (capture) and TZA0100P (detection).
Schematic image of PK bridging ELISA detecting free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Fab format (purple), labeled with HRP.
Fig. 2. Belimumab PK bridging ELISA using antibodies TZA0101 (capture) and TZA0102P (detection).
Schematic image of PK bridging ELISA detecting total drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP. Note for belimumab Type 2 antibodies, the detection antibody is in Fab format.
ADA Bridging ELISA
Fig. 3. Belimumab ADA bridging ELISA using antibodies HCA429, HCA430, and HCA431.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
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