Amyloid Precursor Protein Antibody

Amyloid Precursor Protein Antibody gallery image 1

Western blot analysis of APP in mouse (M) and rat (R) brain tissue lysates with AHP538

Amyloid Precursor Protein Antibody gallery image 2

Immunohistochemical staining of rat brain with Rabbit anti amyloid precursor protein (AHP538)

Amyloid Precursor Protein Antibody gallery image 3

Published customer image:
Rabbit anti Amyloid precursor protein antibody used to detect the amyloid β CT fragment by western blotting.
Image caption:
Western blotting analysis result of BACE1 and ß-CTF. A: bands of BACE1, ß-CTF and ß-tubulin; B: optical density analysis of BACE1; C: optical density analysis of ß-CTF. *: compared with normal control group: p<0.05; #: compared with vehicle control group: p>0.05; ?: compared with normal control group: p<0.05; **: compared with model groups or vehicle control group: p<0.05, n=3.

From: Huang JF, Shang L, Liu P, Zhang MQ, Chen S, Chen D, Fan CL, Wang H, Xiong K. Timosaponin-BII inhibits the up-regulation of BACE1 induced by ferric chloride in rat retina.
BMC Complement Altern Med. 2012 Oct 22;12:189.

Amyloid Precursor Protein Antibody gallery image 4

Published customer image:
Rabbit anti Amyloid precursor protein antibody (AHP538) used for the detection of the amyloid C99 fragment by western blotting of leptomeningeal lysates
Image caption:
Characterization of BACE1 elevation in meningeal cells associated with leptomeningeal amyloidosis. Panel (A) shows BACE1 labeled cells aligning along the pia mater in a case with cerebral amyloid angiopathy with prominent leptomeningeal amyloidosis (B), in contrast to a control case wherein no BACE1 and Aß (6E10) labeling is present at the pia (C,D). Arrows in (A,B) points to profiles with increased BACE1 and 6E10 IR over the background. Panel (E) shows a preparation of leptomeninge sample from the temporal lobe (E), which are rinsed thoroughly in cold phosphate buffer for biochemical analysis (F). Levels of BACE1 protein and C99 in leptomeningeal lysates are elevated in 5 cases with CAA relative to 5 control cases (G,H), as is BACE1 enzymatic activity measured in the lysates (H). Aß42 levels are also higher in the CAA group relative to control (H). In primary cell culture of leptomeningeal biopsies, meningeal cells appear polygonal under phase contrast microscope (J), co-express fibronectin (FN) (K) and BACE1 (L). These cells also express the ß-amyloid precursor protein (APP) (H, the corresponding FN labeling image is not shown). Scale bar?=?250 µm in (A) applying to (B-D), equivalent to 50 µm for inserts in (A,B), 25 µm for (J) and 12.5 µm for (K-M).

From: Xue ZQ, He ZW, Yu JJ, Cai Y, Qiu WY, Pan A, Gai WP, Cai H, Luo XG, Ma C, Yan XX. Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal ß-amyloid deposition in the human brain.
BMC Neurol. 2015 May 2;15:71.

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  • Rabbit anti Amyloid Precursor Protein (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP538P*, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Amyloid Precursor Protein antibody recognizes both intact Amyloid Precursor Protein (APP), and also the C99 fragment generated by Beta-secretase.The sequence recognised by this antibody corresponds to amino acids 85-99 of the C99 fragment. The C99 fragment itself is a substrate for gamma-secretase to generate the 4 kDa beta amyloid peptide, found in the brains of Alzheimer's disease patients.

      APP also inhibits Notch signaling through it’s interaction with NUMB (Roncarati et al. 2002). Rabbit anti Amyloid Precursor Protein antibody has been used successfully for the detection of the ~14 kDa β C-terminal fragment of APP, producted as a result of cleavage by BACE1 using western blotting -in rat retinal lysates (Huang et al.2012).
    • Intended Use
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to APP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Synthetic peptide corresponding to amino acids 737-751 of human APP.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/1000
      Immunohistology - Paraffin(1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin section

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Amyloid Precursor Protein Antibody Formats

    Formats Applications Sizes available
    Amyloid Precursor Protein Antibody : Purified P*, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting


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