Adalimumab Antibody | AbD20349_hIgG1

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Human anti adalimumab specificity ELISA

A microtiter plate was coated over night with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti adalimumab (clone 20349_hIgG1) at a concentration of 2 μg/ml, followed by QuantaBlu™ fluorogenic peroxidase substrate

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Human anti adalimumab specificity titration ELISA

A microtiter plate was coated over night with various antigens. For TNFα/drug complex antigens, human TNFα was immobilized to the plate. After washing and blocking with PBST+5% BSA, adalimumab or infliximab was added. After further incubation and washing steps, detection was performed using HRP-conjugated Human anti adalimumab (clone 20349_hIgG1) titrated to the given concentrations in PBST, followed by QuantaBlu™ fluorogenic peroxidase substrate

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Adalimumab antigen-capture ELISA for PK assay development

A microtiter plate was coated over night with human TNFα at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of adalimumab. Detection was performed using HRP-conjugated Human anti adalimumab (clone 20349_hIgG1) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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  • Human anti Adalimumab (Drug/Target Complex):HRP
  • Human anti Adalimumab (Drug/Target Complex)
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-adalimumab drug-target complex antibody specifically recognizes the adalimumab-TNF alpha complex exclusively and detects adalimumab or biosimilars only when bound to TNF alpha. This affinity-matured antibody is in full IgG1 format and is suitable for a PK antigen capture format assay.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD20349_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA232PEdatasheet pdfdatasheet pdf0.1 mg
      HCA232P
      HCA232Edatasheet pdfdatasheet pdf0.1 mg
      HCA232
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti Adalimumab (drug/target complex), clone AbD20349_hIgG1, is an affinity matured, Type 3 antibody that specifically recognizes adalimumab complexed with TNFα. It does not recognize the free drug or unbound TNFα. Clone AbD20349_hIgG1 can be used in assays to measure adalimumab that has been captured via immobilized TNFα, thereby avoiding the bridging assay format. It can also be used to measure the level of the drug/target complex and biosimilar products using bioanalytical assays.

        Adalimumab (brand name Humira®) is a human monoclonal antibody (IgG1/kappa) approved for the treatment of rheumatoid, psoriatic and juvenile idiopathic arthritis, ankylosing spondylitis, Crohn’s disease and ulcerative colitis. This therapeutic antibody is directed against the pro-inflammatory cytokine tumor necrosis factor alpha (TNFα), blocking its interaction with the p55 (TNFR1, CD120a) and p75 (TNFR2, CD120b) cell surface TNF receptors, resulting in a down-regulation of the abnormal inflammatory response that is characteristic of autoimmune disease.

        View a summary of all anti-adalimumab antibodies.
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This product is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Adalimumab.
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=6.7 nM by real time, label-free molecular interaction analysis on the immobilized adalimumab/TNFα complex.
      • Approx. Protein Concentrations
        Total protein concentration 0.1 mg/ml
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.
        Humira® is a registered trademark of Abbott Laboratories.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.
        Humira® is a registered trademark of Abbott Laboratories.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product is suitable for use in direct ELISA applications.
        Protocol: PK antigen capture ELISA to measure bound drug exclusively
      • ELISA
        This product may be used in a direct ELISA to detect the adalimumab/TNFα complex or to detect adalimumab bound to immobilized TNFα.
        Protocol: PK antigen capture ELISA to measure bound drug exclusively
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Adalimumab Antibody Formats

      Formats Clone Applications Sizes available
      Adalimumab Antibody : Purified AbD20349_hIgG1 E 0.1 mg
      Adalimumab Antibody : HRP AbD20349_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB
            PHP051
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti Adalimumab (Drug/Target Complex)HCA2310.1 mgE
            HCA231
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB
            PHP051

            Recommended Positive Controls

              Histology Controls

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