HCA207 Antigen-Capture Assay using the Adalimumab/TNFa complex binder HCA207 for detection. Human TNFα was used as the coating antigen followed by Adalimumab spiked into 10% human serum. Human anti-Adalimumab/TNFα antibody HCA207 (conjugated to HRP) was used as the detection reagent
Anti-adalimumab drug-target complex antibody specifically recognizes the adalimumab-TNF alpha complex exclusively and detects adalimumab or biosimilars only when bound to TNF alpha. This antibody is in full immunoglobulin format and is suitable for a PK antigen capture format assay.
1 Formats Available
Negative Isotype Controls
Human anti Adalimumab, clone AbD18754_hIgG1, is an antibody that specifically recognizes the adalimumab monoclonal antibody while complexed with TNFα. As clone AbD18754_hIgG1 is a complex-specific antibody that recognizes adalimumab bound to its target, TNFα, it does not recognize either free adalimumab or unbound TNFα.
Clone AbD18754_hIgG1 can be used in assays to measure adalimumab that has been captured via immobilised TNFα. It can also be used to measure adalimumab/TNFα and biosimilar products using bioanalytical assays.
Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
The monovalent intrinsic affinity of this antibody was measured as KD=67 nM by real time, label-free molecular interaction analysis on immobilized adalimumab/TNFα.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline.
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.Humira is a registered trademark of Abbott Laboratories.
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.