Adalimumab Antibody | AbD18754_hIgG1

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HCA207 specificity ELISA using various antigens as coating reagents. HRP conjugated Human anti-Adalimumab antibody (HCA207) was used as the detection reagent

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HCA207 Antigen-Capture Assay using the Adalimumab/TNFa complex binder HCA207 for detection. Human TNFα was used as the coating antigen followed by Adalimumab spiked into 10% human serum. Human anti-Adalimumab/TNFα antibody HCA207 (conjugated to HRP) was used as the detection reagent

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  • Human anti Adalimumab (Drug/Target Complex)
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    Anti-adalimumab drug-target complex antibody specifically recognizes the adalimumab-TNF alpha complex exclusively and detects adalimumab or biosimilars only when bound to TNF alpha. This antibody is in full immunoglobulin format and is suitable for a PK antigen capture format assay.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD18754_hIgG1
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA207Edatasheet pdfdatasheet pdf0.1 mg
      HCA207
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human anti Adalimumab, clone AbD18754_hIgG1, is an antibody that specifically recognizes the adalimumab monoclonal antibody while complexed with TNFα. As clone AbD18754_hIgG1 is a complex-specific antibody that recognizes adalimumab bound to its target, TNFα, it does not recognize either free adalimumab or unbound TNFα.

        Clone AbD18754_hIgG1 can be used in assays to measure adalimumab that has been captured via immobilised TNFα. It can also be used to measure adalimumab/TNFα and biosimilar products using bioanalytical assays.

        Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

        Veiw a summary of all anti-adalimumab antibodies
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Adalimumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=67 nM by real time, label-free molecular interaction analysis on immobilized adalimumab/TNFα.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Humira is a registered trademark of Abbott Laboratories.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA on adalimumab/TNFα complexes or as detection antibody, when conjugated to HRP, for adalimumab bound to TNFα.
        Protocol: PK antigen capture ELISA to measure bound drug exclusively
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Adalimumab Antibody Formats

      Formats Clone Applications Sizes available
      Adalimumab Antibody : Purified AbD18754_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB
            PHP051

            Recommended Positive Controls

              Histology Controls

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