StarBright Dye Publications

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StarBright™ Dyes are novel dyes for flow cytometry designed to have superior brightness, narrow excitation and emission characteristics, ease of use, and stability. Conjugated to highly cited immunology antibodies, the StarBright Dyes range includes dyes excited by the ultraviolet, violet, blue, yellow, and red lasers suitable for use on all cell sorters, cell analyzers, and spectral instruments with the correct lasers and filters.

Peer Reviewed Publications

Here, we have collated all the latest publications citing the use of StarBright Dye conjugated antibodies so you can see how they perform in your hands.

• Carter TH et al. (2024) Delta-9-tetrahydrocannabinol blocks bone marrow-derived macrophage differentiation through elimination of reactive oxygen species. Antioxidants 13, 887
  
  Carter et al (2024) investigated the inhibitory effects of D9-tetrahydrocannabiol (THC) on the differentiation of bone marrow-derived cells into macrophages via the blockade of reactive oxygen species (ROS) production. The authors used StarBright Blue 615 conjugated to F4/80 to identify macrophages

• Gilbert FB and Rainard P. (2024) Expression of the receptor for IgM (FcmR) by bovine neutrophils. Developmental and Comparative Immunology 160 (2024) 105235
  
  Gilbert and Rainard investigated the expression of IgM on bovine neutrophils using anti peptide antibodies based on the sequence. They confirmed bovine neutrophils were able to use IgM to phagocytose and kill bacteria without the help of complement. The authors used StarBright Violet 790 conjugated to CD14 to identify neutrophils.

• Cook SR et al. (2024) Multi-organ immunity on a 3D-printed multi-tissue chip with tubing-free impeller pump bioRxiv 594865
  
  Cook et al developed a 3D-printed, user-friendly multi organ-on -chip device that allowed native tissue structure to be maintained, to co-culture two or more tissue samples under a recirculating common media. Vaccination of their chip resulted in similar antigen accumulation and acute changes in activation markers and gene expression when compared to in vivo vaccination. The authors used StarBright Violet 670 conjugated to CD19 to visualise B cells in microscopy

• Lepland et al. (2024) Therapeutic Tumor Macrophage Reprogramming in Breast Cancer Through a Peptide-Drug Conjugate bioRxiv 607575
  
  Lepland et al investigated the effect of a novel peptide as a useful peptide-drug conjugate to modulate macrophage function in the context of breast tumor immunotherapy. The authors used Ly6C conjugated to StarBright Violet 570, F4/80 conjugated to StarBright Blue 765, CD45 conjugated to StarBright Blue 810 and CD19 conjugated to StarBright Blue 765 to identify various immune cells in the tumor microenvironment by flow cytometry.

• Ferreira et al (2023) Atypical B cells and impaired SARS-CoV-2 neutralization following heterologous vaccination in the elderly Cell Reports 42,112991
  
  Ferreira et al investigated the effect of age on immune response to SARS-CoV-2 vaccination. Multiparameter flow cytometry and single cell RNA sequencing was used to compare the immune response in individuals below 70 and 70 years or older. In older individuals it was found that despite similar levels of spike antibody IgG, there was a reduction in neutralizing antibody potency when receiving a third vaccine dose in addition to sub optimal boosting of spike-specific T cell responses, most markedly for the IL-2 response.

• Roca CP et al. (2023) A cross entropy test allows quantitative statistical comparison of t-SNE and UMAP representations. Cell Reports Methods 3,100390~
  
  Roca et al develop and validate the cross entropy test for robust comparison of dimensionality-reduced datasets in flow cytometry, mass cytometry, and single-cell sequencing, allowing statistical significance assessment and quantification of differences.

• Tucker N et al. (2023). Bovine blood and milk T-cell subsets in distinct states of activation and differentiation during subclinical Staphylococcus aureus mastitis. J Reprod Immunol 156, 103826
  
  This study investigates the differences in T cell populations in bovine blood and milk samples with chronic Staphylococcus aureus infections using a complex flow cytometry panel enabling detection of 15 separate T cell subsets.

• Zheng W et al. (2023). Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during persistent infection. bioRxiv 524758
  
  In this recent study, Zheng et al determined that after T cell responses have developed, CD11c^lo monocyte derived lung cells, termed MNC1 (mononuclear cell subset 1), harbor more live Mycobacterium tuberculosis compared to alveolar macrophages (AM), neutrophils, and less permissive CD11c^hi MNC2. A panel of antibodies, against CD11b, CD11c, Ly6G, Ly6C, I-A/I-E, Siglec F, and F4/80 StarBright Violet 570 (MCA497SBV570), was used to identify the different lung myeloid subsets using flow cytometry.

• Whyte CE et al. (2022). Do more with less: improving high parameter cytometry through overnight staining. Current Protocols 2, e589
  
  Here, Whyte et al demonstrate how increasing antibody incubation times can lead to substantial improvements in sensitivity, maintaining specificity, and reducing background, while also significantly reducing the costs of high-parameter cytometry panels.

Resources and Support

In addition to the publications listed here, you can find StarBright resources as well as a comprehensive set of general flow cytometry resources for self-help. Furthermore, our expert technical support are able to answer any queries including panel designing.