Goat Serum
Normal goat serum (C07SA) used as a blocking reagent to minimise background for immunofluorescence staining of vGLUT1 and VIAAT in rat brain tissue.
Image caption:
Segregated distribution of mCherry-Homer1c (red) and EGFP-gephyrin (green) in interneurons co-transfected by magnetofection at 11 div and processed for immunofluorescence staining for vGluT1 (A) or VIAAT (B) (blue) at 21 div. The boxed areas are enlarged panels A′ and B′. Arrowheads point to postsynaptic clusters apposed to a labeled presynaptic terminal. Arrows indicate isolated, possibly non-synaptic clusters. Scale bars, 20 μm.
From: Bürli T, Baer K, Ewers H, Sidler C, Fuhrer C, Fritschy J-M (2010)
Single Particle Tracking of α7 Nicotinic AChR in Hippocampal Neurons Reveals Regulated Confinement at Glutamatergic and GABAergic Perisynaptic Sites.
PLoS ONE 5(7): e11507.
Normal goat serum (C07SA) used as a blocking reagent to minimise background for immunofluorescence staining of vGLUT1 and VIAAT in rat brain tissue.
Image caption:
Segregated distribution of mCherry-Homer1c (red) and EGFP-gephyrin (green) in interneurons co-transfected by magnetofection at 11 div and processed for immunofluorescence staining for vGluT1 (A) or VIAAT (B) (blue) at 21 div. The boxed areas are enlarged panels A′ and B′. Arrowheads point to postsynaptic clusters apposed to a labeled presynaptic terminal. Arrows indicate isolated, possibly non-synaptic clusters. Scale bars, 20 μm.
From: Bürli T, Baer K, Ewers H, Sidler C, Fuhrer C, Fritschy J-M (2010)
Single Particle Tracking of α7 Nicotinic AChR in Hippocampal Neurons Reveals Regulated Confinement at Glutamatergic and GABAergic Perisynaptic Sites.
PLoS ONE 5(7): e11507.
Normal Goat serum used as a blocking agent to minimize non-specific staining in sections stained for tyrosine hydroxylase and caspase using immunofluorescence.
Image caption:
A proportion of murine TH-IR cells undergo apoptotic changes on postnatal day P2, as identified by immunostaining for activated caspase-3 (a), or by the presence of nuclear condensation (b), white arrow an example of pronounced nuclear condensation. TH-IR cell apoptosis was quantified in the combined SNc and VTA at postnatal day P2 in male and female wild-type (c) or annexin A1 null (d) mice treated with dexamethasone via the maternal drinking water antenatally on embryonic days 16–19 (grey bars 0.5 μg/ml), and compared to the control offspring of dams receiving normal drinking water (open bars). Data are mean ± SEM, n = 6 animals per treatment group. Asterisk a significant effect of treatment, p <0.05 for dexamethasone-treated vs. control animals
From: McArthur S, Pienaar IS, Siddiqi SM, Gillies GE.
Sex-specific disruption of murine midbrain astrocytic and dopaminergic developmental trajectories following antenatal GC treatment.
Brain Struct Funct.221(5):2459-75..
Product Details
- Product Form
- Serum - liquid
- Product Form
- Serum - liquid
- Antiserum Preparation
- Whole serum is supplied 0.2um filtered.
- Preservative Stabilisers
- None Present
- Preservative Stabilisers
0.1% Sodium Azide
Storage Information
- Storage
- Store at -20oC Only.
Storage in frost free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the product. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the product. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Goat Serum
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Functional Assays | |||
| Immunoassay | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoassay | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Goat Serum
-
Bürli, T. et al. (2010) Single particle tracking of alpha7 nicotinic AChR in hippocampal neurons reveals regulated confinement at glutamatergic and GABAergic perisynaptic sites.
PLoS One. 9;5(7):e11507. -
Säve, S. et al. (2011) Activation of Adenosine A2A Receptors Inhibits Neutrophil Transuroepithelial Migration.
Infect Immun. 79: 3431-7. -
Luhmann, U.F. et al. (2014) The severity of retinal pathology in homozygous Crb1rd8/rd8 mice is dependent on additional genetic factors.
Hum Mol Genet. pii: ddu424. [ -
Hippert C et al. (2015) Müller glia activation in response to inherited retinal degeneration is highly varied and disease-specific.
PLoS One. 10 (3): e0120415. -
McArthur, S. et al. (2015) Sex-specific disruption of murine midbrain astrocytic and dopaminergic developmental trajectories following antenatal GC treatment.
Brain Struct Funct. May 6. [Epub ahead of print] -
Herrmann, P. et al. (2015) Cd59a deficiency in mice leads to preferential innate immune activation in the retinal pigment epithelium-choroid with age.
Neurobiol Aging. 36 (9): 2637-48. -
Díaz-Carballo D et al. (2015) Therapeutic potential of antiviral drugs targeting chemorefractory colorectal adenocarcinoma cells overexpressing endogenous retroviral elements.
J Exp Clin Cancer Res. 34: 81.
