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Streptavidin

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Alkaline phosphatase conjugated Streptavidin used for the detection of bound biotinylated Goat anti Human IgA (205008 ) by ELISA.
Image caption:
IgG and IgA responses to whole cell 23F or 6B pneumococci following 23F or 6B challenge, respectively. ELISAs were performed using 23F or 6B pneumococci as targets to measure specific IgG (A, C and E) and IgA (B, D and F) titers in serum (A and B), NW (C and D) and BAL (E and F). Values shown are the mean antibody titers (of triplicates), pre and post 23F (n = 7) or 6B (n = 8) challenge (x-axis). *represents statistical significance between pre- and post-inoculation antibody titers.

From: Wright AKA, Ferreira DM, Gritzfeld JF, Wright AD, Armitage K, Jambo KC, et a.l. (2012)
Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog 8(4): e1002622.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Streptavidin:Alk. Phos.

Product Type
Accessory Reagent
Specificity
Streptavidin

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Alkaline phosphatase conjugated streptavidin is a ~53 kDa protein with a high binding affinity for biotin showing negligable binding to non biotinylated proteins.

Product Form
Streptavidin conjugated to alkaline phosphatase - liquid
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.05%Sodium Azide
1%Bovine Serum Albumin
50%Glycerol
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/10,000
Immunohistology - Frozen 1/50 1/1000
Immunohistology - Paraffin 1/50 1/1000
Western Blotting 1/1000 1/4000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Streptavidin

  1. Wright, A.K. et al. (2012) Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
    PLoS Pathog. 8: e1002622.
  2. Ahmed, U.K. et al. (2016) The Carbohydrate-linked Phosphorylcholine of the Parasitic Nematode Product ES-62 Modulates Complement Activation.
    J Biol Chem. 291 (22): 11939-53.
  3. Pennington, S.H. et al. (2018) Longevity of duodenal and peripheral T-cell and humoral responses to live-attenuated Salmonella typhi. strain Ty21a.
    Vaccine. 36 (31): 4725-33.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"