Streptavidin
Alkaline phosphatase conjugated Streptavidin used for the detection of bound biotinylated Goat anti Human IgA (205008 ) by ELISA.
Image caption:
IgG and IgA responses to whole cell 23F or 6B pneumococci following 23F or 6B challenge, respectively. ELISAs were performed using 23F or 6B pneumococci as targets to measure specific IgG (A, C and E) and IgA (B, D and F) titers in serum (A and B), NW (C and D) and BAL (E and F). Values shown are the mean antibody titers (of triplicates), pre and post 23F (n = 7) or 6B (n = 8) challenge (x-axis). *represents statistical significance between pre- and post-inoculation antibody titers.
From: Wright AKA, Ferreira DM, Gritzfeld JF, Wright AD, Armitage K, Jambo KC, et al. (2012)
Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog 8(4): e1002622.
Alkaline phosphatase conjugated streptavidin is a ~53 kDa protein with a high binding affinity for biotin showing negligable binding to non biotinylated proteins.
Product Details
- Product Form
- Streptavidin conjugated to alkaline phosphatase - liquid
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.05% Sodium Azide 1% Bovine Serum Albumin 50% Glycerol - Approx. Protein Concentrations
- Total protein concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Streptavidin
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2,000 | 1/10,000 | |
| Immunohistology - Frozen | 1/50 | 1/1000 | |
| Immunohistology - Paraffin | 1/50 | 1/1000 | |
| Western Blotting | 1/1000 | 1/4000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Streptavidin
-
Wright, A.K. et al. (2012) Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog. 8: e1002622. -
Ahmed, U.K. et al. (2016) The Carbohydrate-linked Phosphorylcholine of the Parasitic Nematode product ES-62 modulates Complement activation.
J Biol Chem. pii: jbc.M115.702746. [Epub ahead of print]
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
