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Streptavidin

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Streptavidin conjugated to RPE - lyoph thumbnail image 1
Streptavidin conjugated to RPE - lyoph gallery image 1
Detection of biotinylated Goat anti Hamster IgG (STAR79B) in a flow cytometric analysis of EMR1 expression on human eosinophils with Hamster anti Human EMR1(MCA2674) as the primary detection antibody and Streptavidin:RPE (STAR4A) as the tertiary reagent.

Streptavidin:RPE

Product Type
Accessory Reagent
Specificity
Streptavidin

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STAR4A
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Product Form
Streptavidin conjugated to R-Phycoerythrin - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.

STAR4A conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
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  • Select combined or multi-laser view to visualize the spectra

References for Streptavidin

  1. van den Nieuwenhof, I.M. et al. (2001) Differential galactosylation of neuronal and haematopoietic signal regulatory protein-alpha determines its cellular binding-specificity.
    J Cell Sci. 114 (Pt 7): 1321-9.
  2. Tasker, L. & Marshall-Clarke, S. (1997) Immature B cells from neonatal mice show a selective inability to up-regulate MHC class II expression in response to antigen receptor ligation.
    Int Immunol. 9 (4): 475-84.
  3. Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
    J Immunol. 181: 6514-24.
  4. Guesdon, F. et al. (2012) Expression of a glycosylphosphatidylinositol-anchored ligand, growth hormone, blocks receptor signalling.
    Biosci Rep. 32 (6): 653-60.
  5. Nguyen, J. et al. (2021) Quantitative contributions of TNF receptor superfamily members to CD8+ T-cell responses.
    Mol Syst Biol. 17 (11): e10560.

STAR4A

151118

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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