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Streptavidin

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Streptavidin gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Violet 515 (STAR210SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 2
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Blue 700 (STAR210SBB700). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 3
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Violet 440 (STAR210SBV440). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 4
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Biotin conjugated Mouse anti Human CD7 (MCA1197B) detected with Streptavidin StarBright Violet 610 (STAR210SBV610). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 5
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 670 (STAR210SBV670). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 6
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Violet 570 (STAR210SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 7
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Violet 710 (STAR210SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 8
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 790 (STAR210SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 9
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 475 (STAR210SBV475). All experiments performed on red cell lysed human blood gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 10
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright UltraViolet 400 conjugated (STAR210SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 11
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Violet 760 (STAR210SBV760). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 12
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright UltraViolet 665 (STAR210SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 13
Figure A. Starbright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. Starbright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright UltraViolet 445 (STAR210SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 14
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright UltraViolet 795 (STAR210SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 15
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD3 (MCA463SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD3 (MCA463SBV710) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Steptavidin StarBright UltraViolet 510 (STAR210SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 16
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD3 (MCA463SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD3 (MCA463SBV710) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright UltraViolet 575 (STAR210SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 17
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright UltraViolet 605 (STAR210SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 18
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Blue 580 (STAR210SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 19
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD3 (MCA463SBUV400) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Blue 615 (STAR210SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 20
Figure A. PE conjugated Mouse anti Human CD3 (MCA463PE). Figure B. PE conjugated Mouse anti Human CD3 (MCA463PE) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Steptavidin StarBright Blue 675 (STAR210SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 21
Figure A. APC conjugated Mouse anti Human CD3 (MCA463APC). Figure B. APC conjugated Mouse anti Human CD3 (MCA463APC) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Steptavidin StarBright Blue 765 (STAR210SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 22
Figure A. StarBright UltraViolet 605 conjugated Mouse anti Human CD3 (MCA463SBUV605). Figure B. StarBright UltraViolet 605 conjugated Mouse anti Human CD3 (MCA463SBUV605) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Steptavidin StarBright Blue 810 (STAR210SBB810). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 23
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA463SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD3 (MCA463SBUV795) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Steptavidin StarBright Yellow 575 (STAR210SBY575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 24
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765). Figure B. StarBright Blue 765 conjugated Mouse anti Human CD3 (MCA463SBB765) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Yellow 720 (STAR210SBY720). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 25
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Yellow 605 (STAR210SBY605). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 26
Figure A. StarBright Yellow 665 conjugated Mouse anti Human CD3 (MCA463SBV570). Figure B. StarBright Yellow 665 conjugated Mouse anti Human CD3 (MCA463SBV570) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Yellow 665 (STAR210SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 27
Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD3 (MCA463SBY575). Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD3 (MCA463SBY575) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Yellow 800 (STAR210SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 28
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV440) and Biotin conjugated Mouse anti Human CD8β (MCA1226B) detected with Streptavidin StarBright Red 775 (STAR210SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 29
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463 SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463 SBV440) and Biotin conjugated Mouse anti Human CD8 (MCA1226B) detected with Streptavidin StarBright Violet 815 (STAR210SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.Data acquired on the ZE5 Cell Analyzer.
Streptavidin gallery image 30
Figure A. StarBright Blue 580 conjugated Mouse anti Human CD4 (MCA1267SBB580). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD4 (MCA1267SBB580) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright UltraViolet 740 (STAR210SBUV740). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Streptavidin:StarBright Blue 810

Product Type
Accessory Reagent
Specificity
Streptavidin

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STAR210SBB810
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StarBright conjugated Streptavidin secondary reagents are designed for use in flow cytometry in conjunction with biotinylated primary antibodies. Streptavidin:StarBright conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low background.

Product Form
Streptavidin conjugated to StarBright Blue 810 - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Blue 810 477 802
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

STAR210SBB810

100007148

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