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Streptavidin

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Streptavidin:APC

Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
STAR119
Datasheet
F
MSDS
100 Tests loader

APC conjugated Streptavidin is designed as a secondary reagent for use in flow cytometry in conjunction with biotinylated primary antibodies.

APC conjugated Streptavidin conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.

Product Details

Product Form
Streptavidin conjugated to Allophycocyanin - lyophilised
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). Allophycocyanin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Streptavidin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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