Quantum™ R-PE MESF Medium Level

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Quantum™ R-PE MESF Medium Level

Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
20 Tests

100 Tests

Quantum™ R-PE MESF Medium Level is comprised of four calibrated fluorescent populations, with different levels of RPE fluorescence intensity, and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9μm). The excitation and emission spectra of the microspheres match those of cell samples labeled with RPE.

The levels of fluorescence intensity in the different kits (Low, Medium and High) are intended to span the intensity range of common cellular analyses. Medium level kits are used for many types of analyses, and nicely span the range of typical cell samples. Common analyses include those for many surface markers, including CD4 / CD8.

Product Details

Intended Use
FCSC827A is used in the quantitation of RPE fluorescence intensity in Molecules of Equivalent Soluble Fluorochrome (MESF) units. When used in conjunction with Simply Cellular® microspheres this kit also allows quantitation of Antibody Binding Capacity (ABC).

This kit enables fluorescence intensity of a sample to be directly quantified in terms of MESF units and for samples from different instruments to be accurately compared. The fluorescence intensity of each of the five populations in the kit have been calibrated against solutions of laser grade fluorescent dye in units of MESF RPE per microsphere. The Certified Blank population is used to measure the fluorescence detection threshold of the instrument. Correct use of the kit enables:
1) quantitation of fluorescence intensity of samples in terms of MESF;
2) determination of instrument fluorescence detection threshold;
3) determination of instrument linearity;
4) data comparison over time and between multiple instruments.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.01% Gelatin
0.01% Tween 80
Reagents in the Kit
Pack Size: 20 Tests
1 x 1ml bottle of unlabeled microbeads
4 x 1ml bottles of labeled microbeads
Pack Size: 100 Tests
1 x 5ml bottle of unlabeled microbeads
4 x 5ml bottles of labeled microbeads

Storage Information

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guaranteed until date of expiry. Please see product label.

More Information

Quantum™ is a trademark of Bang Laboratories, INC.
For research purposes only

Applications of Quantum™ R-PE MESF Medium Level

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/FCSC827A.pdf

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Quantum™ Simply Cellular® Mouse IgG FCSC815A F 20 Tests
Quantum™ Simply Cellular® Mouse IgG FCSC815B F 100 Tests

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra