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Plate Blocking and Protection Buffer

AbGUARD® Plate Stabilizer

Product Type
Accessory Reagent
Specificity
Plate Blocking and Protection Buffer

Product Code Applications Pack Size List Price Your Price Qty
BUF063
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1000 ml loader
List Price Your Price
loader
BUF063T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml loader
List Price Your Price
loader

Intended Use
BUF063: AbGuard Plate Stabilizer simultaneously stabilizes and preserves microwell plates coated with proteins or other biomolecules and blocks any free binding sites. It preserves the biological activity of bound molecules and prevents degradation, denaturation and leaching. It also blocks free binding sites without creating any interference.
BUF063 is a weakly opaque solution containing proprietary stabilizing components. It does not contain azide, mercury or other toxic compounds. It is biologically inactive.
BUF063T: AbGuard Plate Stabilizer simultaneously stabilizes and preserves microwell plates coated with proteins or other biomolecules and blocks any free binding sites. It preserves the biological activity of bound molecules and prevents degradation, denaturation and leaching. It also blocks free binding sites without creating any interference.
BUF063T is a weakly opaque solution containing proprietary stabilizing components. It does not contain azide, mercury or other toxic compounds. It is biologically inactive.
Product Form
Ready to use Plate Blocking and Protection Buffer - liquid
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4°C. DO NOT FREEZE.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Adsorb the primary protein or other biomolecule to the surface of the microwell according to in-house procedures (typically wells are coated with 100ul of the biomolecule in a fresh carbonate buffer pH 9.6 overnight at 4°C or for 6 hours at room temperature).
2. Without emptying and washing of the plate, add into the coated wells 100-200ul of AbGuard Plate Stabilizer. Saturation is done at room temperature by incubation with AbGuard Plate Stabilizer for 10-15 minutes by gently stirring. Empty the plate by cautious inversion of the plate. DO NOT wash hereafter.
3. Dry the plate for long term storage. Recommended methods are:
• Dry the plate in darkness at room temperature overnight. Do not cover the plate.
• Dry the plate in darkness for 2 - 24 hours in a chamber with low humidity (less than 20%) until totally dry (electronic drying chamber or plastic box containing calcium sulphate or silica gel, etc.). Alternatively, dry for 3 hours at 37°C.
4. Immediately after drying, pack the stabilized microwell plate in an airtight, light-protecting and moisture-proof container or bag with a desiccant.
5. It is recommended to evaluate whether it is necessary to pre-wash stabilized plates prior to use.
Troubleshooting advice: it is recommended to introduce an extra wash step after coating, to remove unbound material, if unexpected results are obtained. Use standard washing methods of inverting the plate and tapping it on an absorbent paper, or use an automatic plate washer. It is essential that the coated area SHOULD NOT be allowed to dry before adding AbGuard Plate Stabilizer.
1. Adsorb the primary protein or other biomolecule to the surface of the microwell according to in-house procedures (typically wells are coated with 100ul of the biomolecule in a fresh carbonate buffer pH 9.6 overnight at 4°C or for 6 hours at room temperature).
2. Without emptying and washing of the plate, add into the coated wells 100-200ul of the AbGuard Plate Stabilizer. Saturation is done at room temperature by incubation with AbGuard Plate Stabilizer for 10-15 minutes by gently stirring. Empty the plate by cautious inversion of the plate. DO NOT wash hereafter.
3. Dry the plate for long term storage. Recommended methods are:
• Dry the plate in darkness at room temperature overnight. Do not cover the plate.
• Dry the plate in darkness for 2 - 24 hours in a chamber with low humidity (less than 20%) until totally dry (electronic drying chamber or plastic box containing calcium sulphate or silica gel, etc.). Alternatively, dry for 3 hours at 37°C.
4. Immediately after drying, pack the stabilized microwell plate in an airtight, light-protecting and moisture-proof container or bag with a desiccant.
5. It is recommended to evaluate whether it is necessary to pre-wash stabilized plates prior to use.
Troubleshooting advice:It is recommended to introduce an extra wash step after coating, to remove unbound material, if unexpected results are obtained. Use standard washing methods of inverting the plate and tapping it on an absorbent paper, or use an automatic plate washer. It is essential that the coated area SHOULD NOT be allowed to dry before adding AbGuard Plate Stabilizer.

BUF063

146547 159598 159599 163413 165303

BUF063T

149741 152663 157425 160426

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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