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Ovalbumin

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Ovalbumin thumbnail image 1 Ovalbumin thumbnail image 2
Ovalbumin gallery image 1
Published customer image:
HRP conjugated ovalbumin (BUF048) used as a detection reagent in a Mouse OVA specific IgE ELISA.
Image caption:
BALF (a) and sera (b) collected from the different mouse groups were assessed for OVA-specific IgE with ELISA. Data are mean ± SD of values from at least 6 mice per group. *Difference from control unchallenged mice, p <0.05; #difference from OVA-challenged mice; p <0.05

From: Ghonim MA, Wang J, Ibba SV, Luu HH, Pyakurel K, Benslimane I, Mousa S, Boulares AH.
Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway.
J Transl Med. 2018 Sep 1;16(1):243.
doi: 10.1186/s12967-018-1621-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Ovalbumin gallery image 2
Published customer image:
HRP conjugated ovalbumin (BUF048) used as a detection reagent in a Mouse OVA specific IgE ELISA.
Image caption:
Mice were subjected to OVA sensitization followed by a single challenge or left unchallenged. Mice were administered, i.p., 10 mg/kg S-NACH or saline 30 min after OVA challenge.
(e) BALF and sera were assessed for OVA-specific IgE with ELISA. Data are mean ± SD of values from at least 6 mice per group. For (e) *difference from control unchallenged mice, p <0.05; #difference from OVA-challenged mice; p <0.05.

From: Ghonim MA, Wang J, Ibba SV, Luu HH, Pyakurel K, Benslimane I, Mousa S, Boulares AH.
Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway.
J Transl Med. 2018 Sep 1;16(1):243.
doi: 10.1186/s12967-018-1621-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Ovalbumin:HRP

Product Type
Accessory Reagent
Specificity
Ovalbumin

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Product Code Applications Pack Size List Price Your Price Qty
BUF048
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
loader

Intended Use
BUF048 is a preparation of HRP conjugated ovalbumin, which may be used for the measurement of ovalbumin specific IgE in immunoassay systems, without species restriction.
Product Form
Purified Ovalbumin conjugated to Horseradish Peroxidase (HRP) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Approx. Protein Concentrations
1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50 1/150
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for Ovalbumin

  1. Hansen, J.S. et al. (2016) Limonene and its ozone-initiated reaction products attenuate allergic lung inflammation in mice.
    J Immunotoxicol. 13 (6): 793-803.
  2. Özkan, M. et al. (2021) Macrophage and dendritic cell subset composition can distinguish endotypes in adjuvant-induced asthma mouse models.
    PLoS One. 16 (6): e0250533.
  3. Ghonim, M.A. et al. (2018) Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway.
    J Transl Med. 16 (1): 243.
  4. Heck, S. et al. (2018) Decreased Migration of Dendritic Cells into the Jugular-Nodose Ganglia by the CXCL12 Neutraligand Chalcone 4 in Ovalbumin-Sensitized Asthmatic Mice.
    Neuroimmunomodulation. 24 (6): 331-40.

BUF048

0414 165921

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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