can be used for preparing cells which will undergo staining for intracellular cytokines or proteins. The Fixation Buffer should be used prior to permeabilization using ICS Perm/Wash Buffer (BUF072
) and has been designed to produce the greatest signal to noise ratio.
- Product Form
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
- Guaranteed until date of expiry. Please see product label.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Fixation Buffer
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Resuspend cells in 0.5 ml Fixation Buffer
2. Leave in dark for 20 min at room temperature
Note: It is recommended that the reagent be titrated for optimal performance for each application
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