Fixation Buffer

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Fixation Buffer

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF071 F 100 ml
Fixation Buffer can be used for preparing cells which will undergo staining for intracellular cytokines or proteins. The Fixation Buffer should be used prior to permeabilization using ICS Perm/Wash Buffer (BUF072) and has been designed to produce the greatest signal to noise ratio.

Product Details

Product Form
Liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Paraformaldehyde

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Shelf Life
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Fixation Buffer

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Resuspend cells in 0.5 ml Fixation Buffer
2. Leave in dark for 20 min at room temperature

Note: It is recommended that the reagent be titrated for optimal performance for each application