Fixation Buffer can be used for preparing cells which will undergo staining for intracellular cytokines or proteins. The Fixation Buffer should be used prior to permeabilization using ICS Perm/Wash Buffer (BUF072) and has been designed to produce the greatest signal to noise ratio.
- Product Form
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
- Shelf Life
- 12 months from date of despatch
- For research purposes only
Applications of Fixation Buffer
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Resuspend cells in 0.5 ml Fixation Buffer
2. Leave in dark for 20 min at room temperature
Note: It is recommended that the reagent be titrated for optimal performance for each application
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