ELISA Neptune Assay Diluent
Neptune ELISA diluent buffer (BUF039) used to dilute serum samples used in an ELISA assay designed to measure gonadotropin-releasing hormone levels in cattle following immunization.
Image caption:
Mean (SD) anti-GnRH antibody titres for cattle treated with the immunocontraceptive vaccine GonaCon. All cattle received a single dose of GonaCon, WB received a booster dose three months later.
From: Massei G, Koon K-K, Benton S, Brown R, Gomm M, Orahood DS, et al. (2015)
Immunocontraception for Managing Feral Cattle in Hong Kong.
PLoS ONE 10(4): e0121598.
ELISA Neptune Assay Diluent is an ELISA assay diluent designed to reduce background noise in problematic human, porcine and bovine serum or plasma samples. Specially formulated for use in antigen-down ELISA, it contains non-mammalian protein and proprietary detergent additives to reduce non-specific IgG adsorption to antigen coated and blocked ELISA well surfaces.
The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF039A equalizes the matrix, inhibits complement and thrombin activity and contains an antimicrobial agent.
The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF039A equalizes the matrix, inhibits complement and thrombin activity and contains an antimicrobial agent.
Product Details
- Product Form
- Ready to use - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of ELISA Neptune Assay Diluent
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Pipette buffer directly onto the plate just prior to adding the sample or standard (suggested volume 50 - 100ul/well).
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| 5x ELISA Coating Buffer | BUF030A | E | 100 ml | ||
| 10x ELISA Wash Buffer | BUF031A | E | 100 ml | ||
| ELISA BSA Block | BUF032A | E | 100 ml | ||
| ELISA Ultrablock | BUF033A | E | 100 ml | ||
| ELISA Ultrablock | BUF033B | E | 500 ml | ||
| ELISA Ultrablock | BUF033C | E | 1000 ml |
Product Specific References
References for ELISA Neptune Assay Diluent
-
Defresne, F. et al. (2010) Differential influence of anticancer treatments and angiogenesis on the seric titer of autoantibody used as tumor and metastasis biomarker.
Neoplasia 12: 562-570. -
Massei, G. et al. (2015) Immunocontraception for managing feral cattle in Hong Kong.
PLoS One. 10 (4): e0121598.
