ELISA Neptune Assay Diluent

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ELISA Neptune Assay Diluent

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF039A E 100 ml
BUF039B E 500 ml
BUF039C E 1000 ml
ELISA Neptune Assay Diluent is an ELISA assay diluent designed to reduce background noise in problematic human, porcine and bovine serum or plasma samples. Specially formulated for use in antigen-down ELISA, it contains non-mammalian protein and proprietary detergent additives to reduce non-specific IgG adsorption to antigen coated and blocked ELISA well surfaces.

The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF039A equalizes the matrix, inhibits complement and thrombin activity and contains an antimicrobial agent.

Product Details

Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of ELISA Neptune Assay Diluent

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Pipette buffer directly onto the plate just prior to adding the sample or standard (suggested volume 50 - 100ul/well).

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
5x ELISA Coating Buffer BUF030A 100 ml E
10x ELISA Wash Buffer BUF031A 100 ml E
ELISA BSA Block BUF032A 100 ml E
ELISA Ultrablock BUF033A 100 ml E
ELISA Ultrablock BUF033B 500 ml E
ELISA Ultrablock BUF033C 1000 ml E

Product Specific References

References for ELISA Neptune Assay Diluent

  1. Defresne, F. et al. (2010) Differential influence of anticancer treatments and angiogenesis on the seric titer of autoantibody used as tumor and metastasis biomarker.
    Neoplasia 12: 562-570.
  2. Massei, G. et al. (2015) Immunocontraception for managing feral cattle in Hong Kong.
    PLoS One. 10 (4): e0121598.