ELISA IgM Reducing Assay Diluent

Product Details
- Intended Use
- BUF038A is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface.
The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF038A equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent. - Product Form
- Ready to use - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.10% Sodium Azide
1-5% Sodium Metabisulphite
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of ELISA IgM Reducing Assay Diluent
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | Neat |
- Instructions For Use
- Pipette buffer directly onto the plate, just prior to adding the sample or standard (suggested volume 50 - 100ul/well).
NB. Since this buffer contains proteins derived from goat, BUF038A is not recommended for ELISA where an anti goat secondary antibody is used.
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
5x ELISA Coating Buffer | BUF030A | E | 100 ml |
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10x ELISA Wash Buffer | BUF031A | E | 100 ml | ||
ELISA BSA Block | BUF032A | E | 100 ml |
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