ELISA IgM Reducing Assay Diluent

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ELISA IgM Reducing Assay Diluent

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF038A E 100 ml
BUF038B E 500 ml

Product Details

Intended Use
BUF038A is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface.

The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF038A equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.
Product Form
Ready to use - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.10% Sodium Azide
1-5% Sodium Metabisulphite

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of ELISA IgM Reducing Assay Diluent

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Pipette buffer directly onto the plate, just prior to adding the sample or standard (suggested volume 50 - 100ul/well).

NB. Since this buffer contains proteins derived from goat, BUF038A is not recommended for ELISA where an anti goat secondary antibody is used.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
5x ELISA Coating Buffer BUF030A 100 ml E
10x ELISA Wash Buffer BUF031A 100 ml E
ELISA BSA Block BUF032A 100 ml E