Cell Freezing Medium
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TC-Protector Cell Freezing Medium
- Product Type
- Accessory Reagent
- Specificity
- Cell Freezing Medium
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| TC-Protector cell freezing mediumis a ready-to-use optimised cryo-preservation medium which allows for the direct freezing of an expansive range of mammalian cell lines at -80°C. TC-Protector cell freezing medium provides a no-mess solution for the protection of cells during frozen storage, and reliably gives a high cell viability and durability rate on thawing. |
- Product Form
- Cell freezing media - liquid
- Preparation
- Chemically defined cryo-preservation medium. Does not contain FBS (foetal bovine serum) or BSA (bovine serum albumin).
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Store unused product at +4oC.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Tissue Culture |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Centrifuge cells pre-suspended in complete growth medium at 1,000-1,500rpm for 60 seconds.
2. Remove supernatant and resuspend cells in TC-Protector at a concentration of 5x106 to 1x107 cells/mL.
3. Transfer this cell suspension to cryogenic storage vial.
4. Freeze vial at -80oC (liquid nitrogen should be used for long-term storage).
References for Cell Freezing Medium
-
Takebe, T. et al. (2014) Transient vascularization of transplanted human adult-derived progenitors promotes self-organizing cartilage.
J Clin Invest. 124 (10): 4325-34.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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