Cell Freezing Medium

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TC-Protector Cell Freezing Medium

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF050B TC 10 X 10ml
BUF050C TC 5 X 10ml
BUF050D TC 1 X 10ml
BUF050A TC 100 ml
TC-Protector cell freezing mediumis a ready-to-use optimised cryo-preservation medium which allows for the direct freezing of an expansive range of mammalian cell lines at -80°C.

TC-Protector cell freezing medium provides a no-mess solution for the protection of cells during frozen storage, and reliably gives a high cell viability and durability rate on thawing.

Product Details

Product Form
Cell freezing media - liquid
Preparation
Chemically defined cryo-preservation medium. Does not contain FBS (foetal bovine serum) or BSA (bovine serum albumin).

Storage Information

Storage
Store unused product at +4oC.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Cell Freezing Medium

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Tissue Culture
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Centrifuge cells pre-suspended in complete growth medium at 1,000-1,500rpm for 60 seconds.

2. Remove supernatant and resuspend cells in TC-Protector at a concentration of 5x106 to 1x107 cells/mL.

3. Transfer this cell suspension to cryogenic storage vial.

4. Freeze vial at -80oC (liquid nitrogen should be used for long-term storage).

Product Specific References

References for Cell Freezing Medium

  1. Takebe, T. et al. (2014) Transient vascularization of transplanted human adult-derived progenitors promotes self-organizing cartilage.
    J Clin Invest. 124 (10): 4325-34.