Avidin/Biotin Blocking System
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The Bio-Rad Avidin/Biotin blocking system is designed to minimise the non-specific staining seen in some tissues due to endogenous avidin, biotin or biotin-binding factors, when using avidin/biotin or streptavidin/biotin detection systems.
Tissue sections should be incubated for 15 minutes with the avidin solution, washed well, and then incubated with the biotin solution for a further 15 minutes. Following further washing the primary antibody may then be added. We recommend that any blocking steps with serum or to block endogenous peroxidase are performed before avidin/biotin blocking.
Tissue sections should be incubated for 15 minutes with the avidin solution, washed well, and then incubated with the biotin solution for a further 15 minutes. Following further washing the primary antibody may then be added. We recommend that any blocking steps with serum or to block endogenous peroxidase are performed before avidin/biotin blocking.
Product Details
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE
This product should be stored undiluted. - Shelf Life
- Please see label for expiry date.
More Information
- Regulatory
- For research purposes only
Applications of Avidin/Biotin Blocking System
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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