Avidin/Biotin Blocking System

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Avidin/Biotin Blocking Reagent

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF016 C P 30 ml
The Bio-Rad Avidin/Biotin blocking system is designed to minimise the non-specific staining seen in some tissues due to endogenous avidin, biotin or biotin-binding factors, when using avidin/biotin or streptavidin/biotin detection systems.

Tissue sections should be incubated for 15 minutes with the avidin solution, washed well, and then incubated with the biotin solution for a further 15 minutes. Following further washing the primary antibody may then be added. We recommend that any blocking steps with serum or to block endogenous peroxidase are performed before avidin/biotin blocking.

Product Details

Preservative Stabilisers
0.1% Sodium Azide (NaN3)

Storage Information

Storage
Store at +4oC. DO NOT FREEZE

This product should be stored undiluted.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Avidin/Biotin Blocking System

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.