The Bio-Rad Avidin/Biotin blocking system is designed to minimise the non-specific staining seen in some tissues due to endogenous avidin, biotin or biotin-binding factors, when using avidin/biotin or streptavidin/biotin detection systems.
Tissue sections should be incubated for 15 minutes with the avidin solution, washed well, and then incubated with the biotin solution for a further 15 minutes. Following further washing the primary antibody may then be added. We recommend that any blocking steps with serum or to block endogenous peroxidase are performed before avidin/biotin blocking.
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Store at +4oC. DO NOT FREEZE
This product should be stored undiluted.
- Guaranteed until date of expiry. Please see product label.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Avidin/Biotin Blocking System
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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