Antigen Retrieval Buffer, pH8.0
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Product Details
- Intended Use
- Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation presented at pH8.0 is suitable for use with most antibodies and gives excellent preservation of tissue morphology.
- Preparation
- Mix 10ml with 90ml distilled water
- Buffer Solution
- 10x
- Buffer Solution
- Ready to use
Storage Information
- Storage
- Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of Antigen Retrieval Buffer, pH8.0
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | |||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Deparaffinize sections and re-hydrate
2. Pre-heat retrieval buffer to 92oC *.
3. Place slides in buffer and incubate 10 minutes at 92oC *.
4. Allow to cool for 5-10 minutes.
5. Rinse in distilled water or PBS, proceed with staining.
* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator. - Instructions For Use
- 1. Deparaffinize sections and re-hydrate
2. Pre-heat retrieval buffer to 92oC *.
3. Place slides in buffer and incubate 10 minutes at 92oC *.
4. Allow to cool for 5-10 minutes.
5. Rinse in distilled water or PBS, proceed with staining.
* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 10x Antigen Retrieval Buffer, pH8.0 | BUF025B | P | 500 ml |
Product Specific References
References for Antigen Retrieval Buffer, pH8.0
-
Chang, J.F. et al. (2011) A possible rare cause of renal failure in streptococcal infection.
Nephrol Dial Transplant. 26 (1): 368-71.
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