Antigen Retrieval Buffer, pH8.0

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Antigen Retrieval Buffer, pH8.0

Antigen Retrieval Buffer, pH8.0

Antigen Retrieval Buffer, pH8.0

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF025B P 500 ml
BUF025A P 500 ml
BUF025C P 50 ml

Product Details

Intended Use
Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation presented at pH8.0 is suitable for use with most antibodies and gives excellent preservation of tissue morphology. Bio-Rad also offers two other versions, an acidic and basic form, which may be of value for use with antibodies that do not work well with this reagent. These buffers may dramatically increase staining intensity but may also have an effect upon tissue morphology.
Preparation
Mix 10ml with 90ml distilled water
Preparation
Mix 10ml with 990ml distilled water
Buffer Solution
10x
Buffer Solution
Ready to use
Buffer Solution
100x

Storage Information

Storage
Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from date of despatch.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Antigen Retrieval Buffer, pH8.0

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin
Immunohistology - Paraffin
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Deparaffinize sections and re-hydrate

2. Pre-heat retrieval buffer to 92oC *.

3. Place slides in buffer and incubate 10 minutes at 92oC *.

4. Allow to cool for 5-10 minutes.

5. Rinse in distilled water or PBS, proceed with staining.

* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.
Instructions For Use
1. Deparaffinize sections and re-hydrate

2. Pre-heat retrieval buffer to 92oC *.

3. Place slides in buffer and incubate 10 minutes at 92oC *.

4. Allow to cool for 5-10 minutes.

5. Rinse in distilled water or PBS, proceed with staining.

* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.
Instructions For Use
1. Deparaffinise sections and re-hydrate

2. Pre-heat retrieval buffer to 92oC *.

3. Place slides in buffer and incubate 10 minutes at 92oC *.

4. Allow to cool for 5-10 minutes.

5. Rinse in distilled water or PBS, proceed with staining.

* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Antigen Retrieval Buffer, pH8.0 BUF025B 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Antigen Retrieval Buffer, pH10.0 BUF026A 500 ml P
Antigen Retrieval Buffer, pH4.8 BUF027A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025B 500 ml P

Product Specific References

References for Antigen Retrieval Buffer, pH8.0

  1. Chang, J.F. et al. (2011) A possible rare cause of renal failure in streptococcal infection.
    Nephrol Dial Transplant. 26 (1): 368-71.