Antigen Retrieval Buffer, pH8.0
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Product Details
- Intended Use
- Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation presented at pH8.0 is suitable for use with most antibodies and gives excellent preservation of tissue morphology. Bio-Rad also offers two other versions, an acidic and basic form, which may be of value for use with antibodies that do not work well with this reagent. These buffers may dramatically increase staining intensity but may also have an effect upon tissue morphology.
- Preparation
- Mix 10ml with 90ml distilled water
- Preparation
- Mix 10ml with 990ml distilled water
- Buffer Solution
- 10x
- Buffer Solution
- Ready to use
- Buffer Solution
- 100x
Storage Information
- Storage
- Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- Please see label for expiry date.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- Please see label for expiry date.
More Information
- Regulatory
- For research purposes only
Applications of Antigen Retrieval Buffer, pH8.0
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | |||
| Immunohistology - Paraffin | |||
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- 1. Deparaffinize sections and re-hydrate
2. Pre-heat retrieval buffer to 92oC *.
3. Place slides in buffer and incubate 10 minutes at 92oC *.
4. Allow to cool for 5-10 minutes.
5. Rinse in distilled water or PBS, proceed with staining.
* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator. - Instructions For Use
- 1. Deparaffinize sections and re-hydrate
2. Pre-heat retrieval buffer to 92oC *.
3. Place slides in buffer and incubate 10 minutes at 92oC *.
4. Allow to cool for 5-10 minutes.
5. Rinse in distilled water or PBS, proceed with staining.
* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator. - Instructions For Use
- 1. Deparaffinise sections and re-hydrate
2. Pre-heat retrieval buffer to 92oC *.
3. Place slides in buffer and incubate 10 minutes at 92oC *.
4. Allow to cool for 5-10 minutes.
5. Rinse in distilled water or PBS, proceed with staining.
* N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025B | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| Antigen Retrieval Buffer, pH10.0 | BUF026A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH4.8 | BUF027A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025B | 500 ml | P |
Product Specific References
References for Antigen Retrieval Buffer, pH8.0
-
Chang, J.F. et al. (2011) A possible rare cause of renal failure in streptococcal infection.
Nephrol Dial Transplant. 26 (1): 368-71.
