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Rat IgG2a Negative Control antibody

Rat IgG2a Negative Control

Product Type
Negative/Isotype Control
Isotype
IgG2a
Specificity
Rat IgG2a Negative Control

Product Code Applications Pack Size List Price Your Price Qty
MCA6005GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
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MCA6005
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 1 mg loader
List Price Your Price
loader

Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Isotype controls savings up to 65%

Target Species
Negative Control
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Trinitrophenol
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

References for Rat IgG2a Negative Control antibody

  1. Barraud-Lange, V. et al. (2020) Partial Sperm beta1 Integrin Subunit Deletion Proves its Involvement in Mouse Gamete Adhesion/Fusion.
    Int J Mol Sci. 21 (22)Nov 11 [Epub ahead of print].
  2. do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
    Research in Veterinary Science. 145: 193-204.
  3. Karayannopoulou, M. et al. (2022) Effect of major versus minor mastectomy on host immunity in canine mammary cancer
    Vet Immunol Immunopathol. Feb 24: 110403.
  4. Matralis, D.T. et al. (2023) Intracellular IFN-γ and IL-4 levels of CD4 + and CD8 + T cells in the peripheral blood of naturally infected (Leishmania infantum) symptomatic dogs before and following a 4-week treatment with miltefosine and allopurinol: a double-blinded, controlled and cross-sectional study.
    Acta Vet Scand. 65 (1): 2.

MCA6005GA

164527 1703

MCA6005

151846

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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