Rat IgG2a Negative Control Antibody

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Rat IgG2a Negative Control Antibody gallery image 1

Figure A. A647 conjugated Mouse anti Canine CD4 (MCA1038A647) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A647 conjugated Mouse anti Canine CD4 (MCA1038A647) and RPE conjugated Mouse anti Canine CD5 (MCA1037PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG2a Negative Control Antibody gallery image 2

Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Mouse anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG2a Negative Control Antibody gallery image 3

Figure A. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. A488 conjugated Rat anti Canine CD44 (MCA1041A488) and RPE conjugated Mouse anti Canine CD45R (MCA1043PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG2a Negative Control Antibody gallery image 4

Figure A. RPE conjugated Rat anti Canine CD45R (MCA1043PE) and A488 conjugated Rat IgG1 isotype control (MCA6005A488). Figure B. RPE conjugated Mouse anti Canine CD45R (MCA1043PE) and A488 conjugated Mouse anti Canine CD44 (MCA1041A488). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat IgG2a Negative Control:Low Endotoxin
  • Rat IgG2a Negative Control:Alexa Fluor® 647
  • Rat IgG2a Negative Control:Alexa Fluor® 488
  • Rat IgG2a Negative Control:Biotin
  • Rat IgG2a Negative Control:APC
  • Rat IgG2a Negative Control:FITC
  • Rat IgG2a Negative Control
  • Rat IgG2a Negative Control:RPE-Cy7
  • Rat IgG2a Negative Control:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
    Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).
    Isotype controls savings up to 65%
    • Product Type
      Negative/Isotype Control
    • Isotype
      IgG2a
    9 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA6005BFdatasheet pdfdatasheet pdf0.1 mg
      MCA6005B
      MCA6005FFdatasheet pdfdatasheet pdf0.1 mg
      MCA6005F
      MCA6005ELFdatasheet pdfdatasheet pdf0.5 mg
      MCA6005EL
      MCA6005Fdatasheet pdfdatasheet pdf1 mg
      MCA6005
      MCA6005APCFdatasheet pdfdatasheet pdf100 Tests
      MCA6005APC
      MCA6005PEFdatasheet pdfdatasheet pdf100 Tests
      MCA6005PE
      MCA6005A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA6005A647
      MCA6005A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA6005A488
      MCA6005PECY7Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA6005PECY7
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human cells in flow cytometry.

        Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
      • Intended Use
      • Target Species
        Negative Control
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE)-Cy7 - lyophilised
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      • Reconstitution
        Reconstitute with 1.0 ml distilled water
        Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
        Reconstitute with 1.0 ml distilled water
        Reconstitute with 1.0 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        None present
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        5% Sucrose
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        5% Sucrose
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        5% Sucrose
      • Immunogen
        Trinitrophenol
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at -20oC only.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC.
        After reconstitution store at +4oC.
        DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC.
        After reconstitution store at +4oC.
        DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
        Prior to reconstitution store at +4oC.
        After reconstitution store at +4oC.
        DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
        Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch
        18 months from date of despatch.
        18 months from date of despatch
        18 months from date of despatch.
        18 months from date of reconstitution
        18 months from date of despatch
        18 months from date of despatch.
        12 months from date of reconstitution
        18 months from date of reconstitution.
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        Cy® and Cydye® are registered trademarks of GE Healthcare
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

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