Rat IgG2a Negative Control Antibody

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Rat IgG2a Negative Control Antibody gallery image 1

Figure A. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat anti mouse CD40 (MCA1143PE). All experiments performed on mouse splenocytes in the presence of mouse SeroBlock (BUF041A)

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Rat IgG2a Negative Control Antibody gallery image 2

Figure A. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat IgG2a isotype control (MCA1212A488). Figure B. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat anti Mouse CD45R (MCA1258A88). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

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Rat IgG2a Negative Control Antibody gallery image 3

Figure A. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat anti Mouse CD48 (MCA925PE). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

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Rat IgG2a Negative Control Antibody gallery image 4

Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Mouse anti Canine CD4 (MCA1038PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG2a Negative Control Antibody gallery image 5

Figure A. RPE conjugated Mouse anti Canine CD5 (MCA1037PE) and A647 conjugated Rat IgG2a isotype control (MCA1212A647). Figure B. RPE conjugated Mouse anti Canine CD5 (MCA1037PE) and A647 conjugated Mouse anti Canine CD4 (MCA1038A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat IgG2a Negative Control Antibody thumbnail image 1
  • Rat IgG2a Negative Control Antibody thumbnail image 2
  • Rat IgG2a Negative Control Antibody thumbnail image 3
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  • Rat IgG2a Negative Control:Preservative Free
  • Rat IgG2a Negative Control:Alexa Fluor® 405
  • Rat IgG2a Negative Control:FITC
  • Rat IgG2a Negative Control:Biotin
  • Rat IgG2a Negative Control:Alexa Fluor® 700
  • Rat IgG2a Negative Control:Alexa Fluor® 647
  • Rat IgG2a Negative Control:Pacific Blue®
  • Rat IgG2a Negative Control:Low Endotoxin
  • Rat IgG2a Negative Control:Alexa Fluor® 488
  • Rat IgG2a Negative Control:RPE-Cy5
  • Rat IgG2a Negative Control:RPE-Alexa Fluor® 647
  • Rat IgG2a Negative Control:RPE
  • Rat IgG2a Negative Control:DyLight® 649
  • Rat IgG2a Negative Control
(Rated 0.0 out of 5 based on 0 customer reviews)
    Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.
    Isotype controls savings up to 65%
    • Product Type
      Negative/Isotype Control
    • Isotype
      IgG2a
    14 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1212BFdatasheet pdfdatasheet pdf0.1 mg
      MCA1212B
      MCA1212D649Fdatasheet pdfdatasheet pdf0.1 mg
      MCA1212D649
      MCA1212ELFdatasheet pdfdatasheet pdf0.5 mg
      MCA1212EL
      MCA1212XZE, Fdatasheet pdfdatasheet pdf1 mg
      MCA1212XZ
      MCA1212E, Fdatasheet pdfdatasheet pdf1 ml
      MCA1212
      MCA1212FFdatasheet pdfdatasheet pdf100 Tests
      MCA1212F
      MCA1212CFdatasheet pdfdatasheet pdf100 Tests
      MCA1212C
      MCA1212PEFdatasheet pdfdatasheet pdf100 Tests
      MCA1212PE
      MCA1212A405Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1212A405
      MCA1212A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1212A700
      MCA1212A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1212A647
      MCA1212PBFdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1212PB
      MCA1212A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1212A488
      MCA1212P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1212P647
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat IgG2a Negative Control antibody is suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells.

        Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with dog cells.

        N.B.This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines.
      • Intended Use
      • Target Species
        Negative Control
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 405 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG conjugated to Pacific Blue® - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE)-Cy5 - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE) Alexa Fluor® 647 - lyophilised
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG conjugated to DyLight®649 - liquid
        Purified IgG - liquid
      • Reconstitution
        Reconstitute with 1 ml distilled water
        Reconstitute with 1 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        None present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        None present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.5%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Immunogen
        Human lymphocytes.
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1mg/ml
        IgG concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
      • Storage
        Store at -20oC.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        6 months from date of despatch.
        12 months from date of reconstitution.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
      • Acknowledgements
        The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        The Pacific Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        Cy® and CyDye® are registered trademarks of GE Healthcare
        This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
        DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
        Flow Cytometry*
        Immunohistology - Frozen
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/10*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry**
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        ELISA
        Flow Cytometry*
        Immunohistology - Frozen

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Streptavidin:APCSTAR119100 TestsF
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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
                  Flow Cytometry

                References

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