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Goat anti Rat Calcitonin Gene-Related Peptide antibody used for the detection of CGRP expressing cells in rat plantar hindpaw skin by immunofluorescence.
Exercise training did not alter hindpaw epidermal innervation or thickness in uninjured or SNI animals. (A, B) 30 μm sections of plantar hindpaw skin were stained with ßIII tubulin (red) and CGRP (red) to quantify total and peptidergic IENF density, respectively. Arrowheads indicate IENF. DAPI (blue) staining was used to visualize the granular cell layer of the epidermis and measure epidermal thickness. (C, D) Both total (βIII tubulin+) and peptidergic (CGRP+) IENF density were unchanged by 1–4 weeks of exercise training in uninjured mice, n = 4–6. Student's t-Test. (E) 25 days after injury, a significant reduction in total (βIII tubulin+) IENF density was observed in SNI, control mice compared to uninjured controls (p<0.0001, n = 4). Exercise training for either 2 or 12 hr per night for 2 weeks did not improve hindpaw epidermal innervation following SNI. One-way ANOVA. (F) Epidermal thickness was unchanged by 1–4 weeks of wheel running in uninjured mice, n = 4–6. Student's t-Test. (G) Compared to uninjured controls, SNI, control hindpaw skin trended towards epidermal thinning that was unaffected by 2 or 12 hr/night of wheel running for 2 weeks, n = 4. One-way ANOVA. In panels E and G, mean and SEM of 2 week, 2 hr/night uninjured, controls are represented by sold and dashed lines, respectively. Data are presented as mean ± SEM. In all statistical analyses, a Bonferroni test was performed to correct for multiple comparisons.
From: Sheahan TD, Copits BA, Golden JP, Gereau RW IV (2015) Voluntary Exercise Training: Analysis of Mice in Uninjured, Inflammatory, and Nerve-Injured Pain States.
PLoS ONE 10(7): e0133191.