CGRP Antibody

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Goat anti Rat calcitonin gene related paptide antibody used for the detection of CRGP expressing axons in rat spinal cord by immunofluorescence.Image caption:
Axon fiber composition in paravertebral ganglia and related labeling in spinal cord. A. Lower power image of two caudal sympathetic ganglia and connecting nerve bridge showing nerve contains a mixture of at least 3 neurochemically distinct fiber populations: HB9-GFP+ sympathetic preganglionic neurons (SPNs), CGRP+ visceral afferents, and TH+ sympathetic postganglionics. Boxed regions are enlarged in B and C. B. Enlargement showing SPNs (GFP+), CGRP and TH immunolabeled axons in axon bundle between two ganglia. Image represents composite of 73 consecutive confocal images taken at 0.38 µm optical section thickness (27.74 µm total thickness). C. Enlargement of SPNs (GFP+), CGRP and TH immunolabeling in the left sympathetic ganglion. Section in central region of ganglia shows that, while SPNs appear to form basket-like synapses around postganglionics, CGRP+ afferents do not project through this region. Image is a collapsed stack of 11 consecutive confocal images (4.18 µm total). D. CGRP (red) and HB9-GFP labeling (green) in a transverse section of thoracic spinal cord. Note predominant CGRP labeling in the dorsal horn. HB9-GFP labeling of SPNs in the intermediolateral nucleus and motoneurons (MNs) in the ventral horn are identified on the right side. Middle panel shows CGRP labeling extending ventrally including into the intermediolateral nucleus (at arrow) while right panel provides a higher magnification image showing CGRP labeling intermingled with SPNs in the intermediolateral nucleus as well as along its medially oriented SPN projections. Scale bar is 100 µm in A, 20 µm in B and C, and 50 µm in D.

From: Zimmerman AL, Sawchuk M, Hochman S (2012) Monoaminergic Modulation of Spinal Viscero-Sympathetic Function in the Neonatal Mouse Thoracic Spinal Cord.
PLoS ONE 7(11): e47213.

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Goat anti calcitonin gene-related peptide antibody used for the detection of CGRP expressing cells by immunofluorescence.
Image caption:
Localization of PKCd and markers of peptidergic and non-peptidergic dorsal root ganglion neurons. A. Representative images of CGRP+, PKCd+, and co-expressing (Merge) DRG neurons. B. Graphical representation of total neurons counted and degree of overlap (n = number of neurons). C, D. Representative images of IB4+, PKCd+, and IB4+/PKCd?+?neurons and illustration of overlap. E, F. Representative images of TRPV1+, PKCd+, and TRPV+/PKCd?+?neurons and illustration of overlap. Inset demonstrates TRPV1 antibody stain in TRPV1-KO mice. (Scale bar?=?50 µm; Arrowheads indicate example cells that express both markers).

From: Valtcheva MV, Davidson S, Zhao C, Leitges M, Gereau RW 4th. Protein kinase Cδ mediates histamine-evoked itch and responses in pruriceptors.
Mol Pain. 2015 Jan 6;11:1.

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Goat anti Rat Calcitonin Gene-Related Peptide antibody used for the detection of CGRP expressing cells in rat plantar hindpaw skin by immunofluorescence.
Image caption:
Exercise training did not alter hindpaw epidermal innervation or thickness in uninjured or SNI animals. (A, B) 30 μm sections of plantar hindpaw skin were stained with ßIII tubulin (red) and CGRP (red) to quantify total and peptidergic IENF density, respectively. Arrowheads indicate IENF. DAPI (blue) staining was used to visualize the granular cell layer of the epidermis and measure epidermal thickness. (C, D) Both total (βIII tubulin+) and peptidergic (CGRP+) IENF density were unchanged by 1–4 weeks of exercise training in uninjured mice, n = 4–6. Student's t-Test. (E) 25 days after injury, a significant reduction in total (βIII tubulin+) IENF density was observed in SNI, control mice compared to uninjured controls (p<0.0001, n = 4). Exercise training for either 2 or 12 hr per night for 2 weeks did not improve hindpaw epidermal innervation following SNI. One-way ANOVA. (F) Epidermal thickness was unchanged by 1–4 weeks of wheel running in uninjured mice, n = 4–6. Student's t-Test. (G) Compared to uninjured controls, SNI, control hindpaw skin trended towards epidermal thinning that was unaffected by 2 or 12 hr/night of wheel running for 2 weeks, n = 4. One-way ANOVA. In panels E and G, mean and SEM of 2 week, 2 hr/night uninjured, controls are represented by sold and dashed lines, respectively. Data are presented as mean ± SEM. In all statistical analyses, a Bonferroni test was performed to correct for multiple comparisons.

From: Sheahan TD, Copits BA, Golden JP, Gereau RW IV (2015) Voluntary Exercise Training: Analysis of Mice in Uninjured, Inflammatory, and Nerve-Injured Pain States.
PLoS ONE 10(7): e0133191.

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Goat anti Rat Calcitonin Gene-Related Peptide antibody used for the detection of CGRP expressing axons in rat spinal cord sections by immunofluorescence.
Image caption:
Effects of DFO on scarring and regeneration. Tissue preservation, lesion size and axon regeneration at 19 dpl. (A) Exemplary mosaic compositions of PBS- (A) and DFO- (B) treated spinal cord stained with anti-GFAP to visualize the GFAP-negative scar area. Lesion area marked with dotted lines, Asterisks indicate a cystic area in both treatment groups. S3 Fig provides pictures A and B with higher intensity to show that the lesion site is filled with tissue. Straight lines outline the region of interest used for quantification of lesion site (2.5 mm). DFO animals had significantly smaller lesion areas (corrected for the total area) than PBS controls (C) and significantly more tissue sparing (D) and. (E) Quantification of the number of CST axons per mm2 of lesion area revealed a significant increase. (F) the CST tracing was equal in both groups. (G) The number of CGRP axons in the scar was significantly increased in DFO-treated rats. (H, I) Representative pictures of CGRP axon profiles (arrows) in the lesion area in DFO- and PBS-treated animals. Note that the red spots in I originate from aberrantly stained macrophages. Statistics: unpaired T-test * p < 0.05, ** p < 0.01. Scale bar (G, H) = 50 μm.

From: Vogelaar CF, König B, Krafft S, Estrada V, Brazda N, Ziegler B, et al. (2015) Pharmacological Suppression of CNS Scarring by Deferoxamine Reduces Lesion Volume and Increases Regeneration in an In Vitro Model for Astroglial-Fibrotic Scarring and in Rat Spinal Cord Injury In Vivo. PLoS ONE 10(7): e0134371.

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  • Goat anti Rat Calcitonin Gene-Related Peptide
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1720-9007C, E, IF, Pdatasheet pdfdatasheet pdf0.1 ml
    1720-9007
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Rat Calcitonin Gene-Related Peptide antibody recognizes Calcitonin gene-related peptide, also known as CGRP, a neuropeptide that acts as a vasodilator and may play a role in migraine. Goat anti Rat Calcitonin Gene-Related Peptide antibody reacts with both the whole molecule (amino acids 1-37) and the C-terminal fragment (23-37).
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Guinea Pigyes
      Emuyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified Ig - liquid
    • Reconstitution
    • Preparation
      Purified Ig prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Synthetic rat Tyr-CGRP (23-37) conjugated to gamma globulin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      extracellular region
      hormone activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/2500
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunohistology - Resin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CGRP Antibody Formats

    Formats Applications Sizes available
    CGRP Antibody : Purified C, E, IF, P 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Immunohistology - Paraffin

              References

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