Rabbit Complement
Rabbit Complement (C12CC) used as asource of complement in a complemnt dependent cytotoxicity assay on T-cell prolymphocytic leukemia cells.
Image caption:
Anti-CCR7 mAb mediates a strong CDC on T-PLL cells. a CDC on alemtuzumab-naïve patients. Leukemic cells from T-PLL patients (n = 8) were incubated with anti-CCR7, anti-CD52 (alemtuzumab), or an IC at the indicated concentrations and then exposed to rabbit complement for 1.5 h. The percentage of specific cell lysis was determined through quantification of 7-AAD incorporation by flow cytometry. b CDC on alemtuzumab-refractory patients. Specific lysis on T-PLL cells from 4 refractory patients was assayed as described in A.
From: Cuesta-Mateos C, et al.
CCR7 as a novel therapeutic target in t-cell PROLYMPHOCYTIC leukemia.
Biomark Res. 2020 Oct 24;8:54.
doi: 10.1186/s40364-020-00234-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit Complement
- Product Type
- Serum
- Specificity
- Rabbit Complement
Antibody Quality and Performance Guarantee
| This rabbit serum is intended for use as a source of rabbit complement for cytotoxicity investigations and HLA-ABC tissue typing. This material will appear cloudy after reconstitution. the cloudy appearance is a result of the lyophilation process, and does not affect potency. Use within one hour of reconstitution, keeping on ice throughout. |
- Product Form
- Rabbit serum - lyophilized.
- Reconstitution
- Reconstitute with 2 ml ice cold distilled water.
- Preservative Stabilisers
- None present.
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC for 1 hour or aliquot and store at -70oC for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the product.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the product.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Functional Assays 1 |  |
||
| Immunoassay | |
- 1 This product is not sold as sterile but can be sterilized by filtration if necessary. It is preferable to dilute the complement to a final working concentration before filtration in order to minimize loss of volume.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Rabbit Complement
-
(1983) Essential aspects of tissue typing: Preparation, use, storage of reagents and standardisation of complement. Council of Europe. European Health Committee, Strasbourg. ISBN 92-871.224-4.
-
(1979) NIAID Manual of tissue typing techniques. Edited by J.G. Ray. Publication No. NIH-80-545.
-
Cuesta-Mateos, C. et al. (2020) CCR7 as a novel therapeutic target in t-cell PROLYMPHOCYTIC leukemia.
Biomark Res. 8: 54.
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