Kinetoplastid Membrane Protein 11 Antibody | L-157

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  • Mouse anti Kinetoplastid Membrane Protein 11
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  • Product Type
    Monoclonal Antibody
  • Clone
    L-157
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT2003E, IB, IFdatasheet pdfdatasheet pdf0.5 ml
    OBT2003
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 recognizes Kinetoplastid Membrane Protein-11 (KMP-11), previously known as the lipophosphoglycan-associated protein (LPGAP), a 92 amino acid ~11 kDa membrane protein. Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 was originally derived against Leishmania donovani lipophosphoglycan, but found to recognize the tightly associated LPGAP. KMP-11 is found in a wide variety of Kinetoplastid parasites including African trypanosomes, Leishmania and Crithidia (Tolson et al. 1994).
    • Intended Use
    • Target Species
      Protozoan
    • Product Form
      Ascites - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Preservative Stabilisers
      None present
    • Immunogen
      L. donovani lipophosphoglycan semi purified from epimastigotes.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      cytoskeleton organization
      cytoplasm
      positive regulation of cell proliferation
      defense response
      microtubule
      T cell proliferation
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2000
      Immunoblotting1/10001/3000
      Immunofluorescence1/5001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Kinetoplastid Membrane Protein 11 Antibody Formats

    Formats Clone Applications Sizes available
    Kinetoplastid Membrane Protein 11 Antibody : Ascites L-157 E, IB, IF 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Source Reference

              References

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