Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 recognizes Kinetoplastid Membrane Protein-11 (KMP-11), previously known as the lipophosphoglycan-associated protein (LPGAP), a 92 amino acid ~11 kDa membrane protein. Mouse anti Kinetoplastid Membrane Protein 11 antibody, clone L-157 was originally derived against Leishmania donovani lipophosphoglycan, but found to recognize the tightly associated LPGAP. KMP-11 is found in a wide variety of Kinetoplastid parasites including African trypanosomes, Leishmania and Crithidia (Tolson et al. 1994).
Ascites - lyophilised
Reconstitute with 0.5 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
L. donovani lipophosphoglycan semi purified from epimastigotes.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Instructions For Use
Additional Kinetoplastid Membrane Protein 11 Antibody Formats
1. Tolson, D.L. et al. (1989) The immunochemical structure and surface arrangement of Leishmania donovani lipophosphoglycan determined using monoclonal antibodies.Mol Biochem Parasitol. 35 (2): 109-18.
1. Tolson, D.L. et al. (1994) The kinetoplastid membrane protein 11 of Leishmania donovani and African trypanosomes is a potent stimulator of T-lymphocyte proliferation.Infect Immun. 62 (11): 4893-9.
2. Pearson, T.W. et al. (2000) The major cell surface glycoprotein procyclin is a receptor for induction of a novel form of cell death in African trypanosomes in vitro.Mol Biochem Parasitol. 111 (2): 333-49.