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Histidine Rich Protein II Antibody | 14971

Histidine Rich Protein II Antibody | 14971 gallery image 1

Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from Plasmodium falciparum) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium FalciparumHRPII (HCA159) antibody

  • Histidine Rich Protein II Antibody | 14971 thumbnail image 1
  • Human anti Plasmodium falciparum HRPII
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA160Edatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • HCA160 recognises HRPII (Histidine Rich Protein II) from the Malaria causing protozoan species Plasmodium falciparum.

      Clone 14971 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

      HCA160 is designed for use as a capture antibody with HCA159P as the detection antibody in sandwich assay development.
    • Intended Use
    • Target Species
    • Product Form
      Human IgG1 antibody selected from the HuCAL ® phage display library and expressed in a human cell line. This product is supplied as liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant HRPII fusion protein from Plasmodium falciparum
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
      This product is suitable for use in indirect ELISA applications. This product may be used as a capture reagent in a sandwich ELISA together with HCA159P as the detection reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Histidine Rich Protein II Antibody Formats

    Formats Clone Applications Sizes available
    Histidine Rich Protein II Antibody : Purified 14971 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Histidine Rich Protein II Antibody | 14971 thumbnail image 1

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