TNF Alpha
Recombinant Sheep TNF Alpha
- Product Type
- Recombinant Protein
- Specificity
- TNF Alpha
Antibody Quality and Performance Guarantee
| Recombinant Sheep Tumor Necrosis Factor (TNF) alpha is a recombinant protein corresponding to aa 78-234 of native sheep TNF alpha. TNF alpha is produced by activated macrophages, monocytes, endothelial cells, fibroblast and both T and B lymphocytes. Production of ovine TNF alpha can be induced by a variety of factors, dependant upon cell type, and includes bacterial toxins, IL-1, PDGF, IFN beta, NGF, oncostatin M and viral infections. TNF alpha functions in the regulation of immune cells, is able to induce fever, apoptotic cell death, sepsis, cacheria, inflammation and to inhibit tumorigenesis and viral replication. |
- Target Species
- Sheep
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 17.2 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P23383
- Entrez Gene
- TNF
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
PSP005
153967
162628
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with TNF ALPHA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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