TNF Alpha

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Recombinant Rat TNF Alpha

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PRP16 E FN WB 20 µg
Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells - this batch demonstrated an ED50 of <0.05ng/ml.

Product Details

Target Species
Rat
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant rat TNF alpha purified from E.coli.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Activity
>2 x 107 units/mg
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution.
Protein Molecular Weight
17.3kD (157 Amino acid sequence)
Endotoxin Level
<0.1ng/ug

Storage Information

Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P16599 Related reagents
Entrez Gene
Tnf Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0001775 cell activation
GO:0002037 negative regulation of L-glutamate transport
GO:0003009 skeletal muscle contraction
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0009612 response to mechanical stimulus
GO:0019722 calcium-mediated signaling
GO:0008285 negative regulation of cell proliferation
GO:0042493 response to drug
GO:0014823 response to activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043525 positive regulation of neuron apoptosis
GO:0045840 positive regulation of mitosis
GO:0046325 negative regulation of glucose import
GO:0050806 positive regulation of synaptic transmission
Regulatory
For research purposes only

Applications of TNF Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4 ng/well
Functional Assays
Western Blotting 1.5 3.0 ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a standard in ELISA applications with either AAR33 or AAR33B.
Western Blotting
This product may be used as the posititve control in Western Blotting applications with either AAR33 or AAR33B.

Product Specific References

References for TNF Alpha

  1. Rayner, S.A. et al. (2000) Local bioactive tumour necrosis factor (TNF) in corneal allotransplantation.
    Clin Exp Immunol. 122 (1): 109-16.
  2. Lee, P.T. et al. (2000) Role of alveolar macrophages in innate immunity in neonates: evidence for selective lipopolysaccharide binding protein production by rat neonatal alveolar macrophages.
    Am J Respir Cell Mol Biol. 23 (5): 652-61.
  3. Dumont, A.O. et al. (2014) Differential Regulation of Glutamate Transporter Subtypes by Pro-Inflammatory Cytokine TNF-α in Cortical Astrocytes from a Rat Model of Amyotrophic Lateral Sclerosis.
    PLoS One. 9: e97649.