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Phosphorylase B

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Native Rabbit Phosphorylase B

Product Type
Purified Protein
Specificity
Phosphorylase B

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Native Rabbit Phosphorylase B is a member of the protein kinase superfamily. Phosphorylases catalyse the breakdown of glycogen in liver and muscle. The phosphorylase of skeletal muscle occurs in two forms, the active form (phosphorylase-a) and a much less active form (phosphorylase b).
Phosphorylase b has a molecular weight of approximately 185kDa.

Target Species
Rabbit
Product Form
Purified enzyme from rabbit skeletal muscle - liquid
Preservative Stabilisers
None present
Activity
55-60 U/mg protein

One unit will liberate one µM of glucose-1-phosphate from glycogen and orthophosphate per minute at 37ºC and pH 6.8.
Purity
Protein: 95%
Approx. Protein Concentrations
5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Further Reading

  1. Fischer, E.H. and Krebs, E.G. (1958) The isolation and crystallization of rabbit skeletal muscle phosphorylase b.
    J Biol Chem. 231: 65-71.
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