Phosphorylase B
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Native Rabbit Phosphorylase B is a member of the protein kinase superfamily. Phosphorylases catalyse the breakdown of glycogen in liver and muscle. The phosphorylase of skeletal muscle occurs in two forms, the active form (phosphorylase-a) and a much less active form (phosphorylase b).
Phosphorylase b has a molecular weight of approximately 185kDa.
Phosphorylase b has a molecular weight of approximately 185kDa.
Product Details
- Target Species
- Rabbit
- Product Form
- Purified enzyme from rabbit skeletal muscle - liquid
- Preservative Stabilisers
- None present
- Activity
- 55-60 U/mg protein
One unit will liberate one µM of glucose-1-phosphate from glycogen and orthophosphate per minute at 37ºC and pH 6.8. - Purity
- Protein: 95%
- Approx. Protein Concentrations
- 5.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Phosphorylase B
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
Further Reading
-
Fischer, E.H. and Krebs, E.G. (1958) The isolation and crystallization of rabbit skeletal muscle phosphorylase b.
J Biol Chem. 231: 65-71.
