Native Rabbit Phosphorylase B is a member of the protein kinase superfamily. Phosphorylases catalyse the breakdown of glycogen in liver and muscle. The phosphorylase of skeletal muscle occurs in two forms, the active form (phosphorylase-a) and a much less active form (phosphorylase b).
Phosphorylase b has a molecular weight of approximately 185kDa.
- Target Species
- Product Form
- Purified enzyme from rabbit skeletal muscle - liquid
- Preservative Stabilisers
- None present
- 55-60 U/mg protein
One unit will liberate one µM of glucose-1-phosphate from glycogen and orthophosphate per minute at 37ºC and pH 6.8.
- Protein: 95%
- Approx. Protein Concentrations
- 5.0 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Phosphorylase B
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
Product Specific References
Fischer, E.H. and Krebs, E.G. (1958) The isolation and crystallization of rabbit skeletal muscle phosphorylase b.
J Biol Chem. 231: 65-71.