IL-8

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Recombinant Rabbit Interleukin-8

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PRABP006 Datasheet	E WB SDS	5 µg	Log in		Get Quote

Summary
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Recombinant Rabbit interleukin-8 (IL-8) is a recombinant protein corresponding to aa 23-101 of native rabbit IL-8.

Rabbit IL-8, also known as CXCL8, is a chemokine secreted by a range of cell types including macrophages and endothelial cells in response to infection and functions as a chemotactin on T lymphocytes, neutrophils and basophils.

Product Details

Target Species: Rabbit
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10% Trehalose
Purity: >95% by SDS PAGE analysis
Protein Molecular Weight: 9.1 kDa

Storage Information

Storage: Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Guarantee: 12 months from date of despatch

More Information

UniProt: P19874
Entrez Gene: IL8
GO Terms: GO:0005153 interleukin-8 receptor binding; GO:0005615 extracellular space; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0007050 cell cycle arrest; GO:0008009 chemokine activity; GO:0030155 regulation of cell adhesion; GO:0030593 neutrophil chemotaxis; GO:0045091 regulation of retroviral genome replication; GO:0050930 induction of positive chemotaxis
Regulatory: For research purposes only

Applications of IL-8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2992 as a coating antibody and AHP2992B as a detection antibody.

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