IL-8
Recombinant Rabbit interleukin-8 (PRABP006) detected using purified Goat anti Rabbit interleukin-8 (AHP2992) as the capture reagent and biotinylated Goat anti Rabbit interleukin-8 (AHP2992B) as the detection reagent followed by horseradish peroxidase conjugated Streptavidin (STAR5B)
Recombinant Rabbit interleukin-8 (IL-8) is a recombinant protein corresponding to aa 23-101 of native rabbit IL-8.
Rabbit IL-8, also known as CXCL8, is a chemokine secreted by a range of cell types including macrophages and endothelial cells in response to infection and functions as a chemotactin on T lymphocytes, neutrophils and basophils.
Rabbit IL-8, also known as CXCL8, is a chemokine secreted by a range of cell types including macrophages and endothelial cells in response to infection and functions as a chemotactin on T lymphocytes, neutrophils and basophils.
Product Details
- Target Species
- Rabbit
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 9.1 kDa
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P19874 Related reagents
- Entrez Gene
- IL8 Related reagents
- GO Terms
- GO:0005153 interleukin-8 receptor binding
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007050 cell cycle arrest
- GO:0008009 chemokine activity
- GO:0030155 regulation of cell adhesion
- GO:0030593 neutrophil chemotaxis
- GO:0045091 regulation of retroviral genome replication
- GO:0050930 induction of positive chemotaxis
- Regulatory
- For research purposes only
Applications of IL-8
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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