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E 500 mg loader
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Partially purified porcine pancreatic trypsin, a serine protease that shows preferential cleavage at Arg-|-Xaa, and Lys-|-Xaa sites.

Target Species
Product Form
Partially purified enzyme from porcine pancreas - liquid
Preservative Stabilisers
None present
8576 U/mg solid

1 unit causes an increase in absorbance of 0.0003 per minute at 25ºC and 253 nm, pH 7.6 on the substrate benzoyl-L arginine ester (BAEE).
Approx. Protein Concentrations
Total protein concentration 100 mg/ml
For research purposes only
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0046872 metal ion binding
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0007586 digestion


010715 157187 159354 163808 164940

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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