MCP-1
Recombinant Pig MCP-1 (PPP016) detected using Rabbit anti Pig MCP-1 as the capture reagent and biotinylated Rabbit anti Pig MCP-1 antibody as the detection reagent followed by Streptavidin:HRP
Recombinant Pig MCP-1
- Product Type
- Recombinant Protein
- Specificity
- MCP-1
Antibody Quality and Performance Guarantee
| Recombinant Pig MCP-1 is an 8.6 kDa peptide corresponding to aa 24 – 99 of porcine monocyte chemotactic protein 1 (MCP-1). Also known as CCL2 , MCP-1 is a secreted chemokine involved in the recruitment of monocytes to sites of injury or inflammation. MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages. |
- Target Species
- Pig
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 8.6 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for MCP-1
-
Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
Res Vet Sci. 131: 266-300.
- Synonyms
- CCL2
- UniProt
- P42831
- Entrez Gene
- CCL2
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
PPP016
136889
163809
167660
169274
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with MCP-1 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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