IL-17A

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Recombinant Pig Interleukin-17A

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PPP028 E WB 5 µg
Recombinant pig interleukin-17A (IL-17A) is a recombinant protein corresponding to aa 24-153 of native porcine IL-17A.

Native porcine IL-17A is a 17.3 kDa regulatory cytokine belonging to the IL-17 cytokine family. Porcine IL-17A is produced primarily in activated T cells and is found at high levels in porcine intestinal tissues including the jejunum and ileum. Porcine IL-17A functions in the mediation of proinflammatory and induces the production of other cytokines.

Product Details

Target Species
Pig
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
14.9 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q60I29 Related reagents
Entrez Gene
IL17A Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
Regulatory
For research purposes only

Applications of IL-17A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2395 as a coating antibody and AHP2395B as a detection antibody.