IL-10

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Recombinant Pig Interleukin-10

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
PPP025	E WB			5 µg

Summary

Recombinant Pig interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted anti inflammatory cytokine produced primarily by monocytes. This product is a purified recombinant cytokine corresponding to aa 18-175 of porcine IL-10. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα.

Product Details

Target Species: Pig
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10% Trehalose
Purity: >95% by SDS PAGE analysis
Protein Molecular Weight: 18.1 kDa

Storage Information

Storage: Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life: 12 months from date of despatch

More Information

UniProt: Q29055 Related reagents
Entrez Gene: IL10 Related reagents
GO Terms: GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0006955 immune response
Regulatory: For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2393 as a coating antibody and AHP2393B as a detection antibody.

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