IL-10
Recombinant Pig Interleukin-10
- Product Type
- Recombinant Protein
- Specificity
- IL-10
Antibody Quality and Performance Guarantee
| Recombinant Pig interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted anti inflammatory cytokine produced primarily by monocytes. This product is a purified recombinant cytokine corresponding to aa 18-175 of porcine IL-10. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα. |
- Target Species
- Pig
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 18.1 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IL-10
-
Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
Res Vet Sci. 131: 266-300.
- Synonyms
- Cytokine Stimulating Inhibitory Factor
- UniProt
- Q29055
- Entrez Gene
- IL10
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
PPP025
156776
164720
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-10 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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