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IL-10

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Pig Interleukin-10 ELISA

Recombinant Pig interleukin-10 (PPP025) detected using Rabbit anti Pig interleukin-10 (AHP2393) as the capture reagent and Rabbit anti Pig interleukin-10:Biotin (AHP2393B) as the detection reagent followed by Streptavidin:HRP.

Recombinant Pig Interleukin-10

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PPP025
Datasheet
E WB
MSDS
5 µg loader

Recombinant Pig interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted anti inflammatory cytokine produced primarily by monocytes. This product is a purified recombinant cytokine corresponding to aa 18-175 of porcine IL-10. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IFNγ, IL-2, IL-4, GM-CSF and TNFα.

Product Details

Target Species
Pig
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
18.1 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Guarantee
12 months from date of despatch

More Information

UniProt
Q29055 Related reagents
Entrez Gene
IL10 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IL-10

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2393 as a coating antibody and AHP2393B as a detection antibody.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
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