Erythropoietin

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Recombinant Pig Erythropoietin

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PPP021 E WB 5 µg
Recombinant Pig erythropoietin is an 18.6 kDa recombinant protein corresponding to aa 27 - 194 of native porcine erythropoietin (EPO).

Erythropoietin, commonly known as EPO or hematopoietin, is a secreted protein belonging to the EPO/TPO family and involved in erythrocyte regulation.

Erythropoietin is a hormone secreted from, in adult mammals, primarily the kidney but to a lesser extent the liver. In the fetus, EPO is secreted from the liver. EPO functions as a regulatory cytokine in the differentiation of erythrocytes.

Product Details

Target Species
Pig
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trahalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
18.6 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life
12 months from date of despatch

More Information

UniProt
P49157 Related reagents
Entrez Gene
EPO Related reagents
GO Terms
GO:0005128 erythropoietin receptor binding
GO:0005179 hormone activity
GO:0043249 erythrocyte maturation
Regulatory
For research purposes only

Applications of Erythropoietin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2389 as a coating antibody and AHP2389B as a detection antibody.