Erythropoietin
Recombinant Pig Erythropoietin
- Product Type
- Recombinant Protein
- Specificity
- Erythropoietin
Antibody Quality and Performance Guarantee
| Recombinant Pig erythropoietin is an 18.6 kDa recombinant protein corresponding to aa 27 - 194 of native porcine erythropoietin (EPO). Erythropoietin, commonly known as EPO or hematopoietin, is a secreted protein belonging to the EPO/TPO family and involved in erythrocyte regulation. Erythropoietin is a hormone secreted from, in adult mammals, primarily the kidney but to a lesser extent the liver. In the fetus, EPO is secreted from the liver. EPO functions as a regulatory cytokine in the differentiation of erythrocytes. |
- Target Species
- Pig
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 18.6 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Erythropoietin
-
Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
Res Vet Sci. 131: 266-300.
- UniProt
- P49157
- Entrez Gene
- EPO
- GO Terms
- GO:0005128 erythropoietin receptor binding
- GO:0005179 hormone activity
- GO:0043249 erythrocyte maturation
View more products with ERYTHROPOIETIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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