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IgG

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Purified Mouse IgG thumbnail image 1 Purified Mouse IgG thumbnail image 2
Purified Mouse IgG gallery image 1
Published customer image:
Purified Mouse IgG used for injection into rats to monitor antibody deposition in pripheral nerve tissues by immunofluorescence
Image caption:
Antibodies accumulate in peripheral nerves after passive transfer and lead to macrophage increase. (A) Immunohistochemical localization of antibodies after systemic injection of IgG in the femoral quadriceps (q) and saphenous nerve (s) from 6-month-old P0wt JHD−/− (7 days post injection, DPI) and P0het JHD−/− mice (7DPI and 28DPI). Note stronger IgG immunoreactivity in the endoneurium from P0het JHD−/− mice that received consecutive IgG injections over 28 days (28DPI). Scale bar, 20 μm. (B) Quantification of F4/80-positive profiles in non-injected control and reconstituted mice showing an increase of macrophage numbers in femoral quadriceps nerve from P0het JHD−/− mutants after IgG reconstitution (n = 3 to 5). One-way ANOVA with Bonferroni-Holm correction. *P < 0.05. (C) Quantification of foamy macrophages reveals an increase of phagocytosing macrophages in femoral quadriceps nerves from P0het JHD−/− mice after IgG reconstitution. Note also the presence of phagocytosing macrophages in control-reconstituted animals (+KLH, +IgG Fc) (n = 3 to 5). Kruskal-Wallis test with Bonferroni-Holm correction. *P < 0.05. n.d. (not detected).

From: Klein, D., Groh, J., Weishaupt, A., & Martini, R. (2015). Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B.
Journal of Neuroinflammation, 12, 49.
Purified Mouse IgG gallery image 2
SDS-Page of Mouse IgG whole molecule Lane 1: Mouse IgG reduced Lane 3: Mouse IgG non-reduced Load: 1 µg per lane Predicted/Observed size (non-reduced): 160 kDa, 160 kDa Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa

Purified Mouse IgG

Product Type
Purified Protein
Product Code Applications Pack Size List Price Quantity
PMP01
Datasheet
E IY
SDS
2 mg loader

PMP01X
Datasheet
E IY
SDS
10 mg loader

Secreted by mature B cells, IgG are the most abundant immunoglobulin isotype and are major components of the adaptive immune system. IgG are found in all body fluids where they recognize and bind antigenic determinants exposed at the surface of invading pathogens to neutralize pathogens and activate other defense cells and the complement system. This Mouse IgG preparation has been prepared from normal mouse serum and purified by ion exchange chromatography, but may contain traces of other immunoglobulin class. By immunoelectrophoresis a single band is seen against anti-Mouse IgG and anti-Mouse serum. Trace amounts of other immunoglobulins present may be detected if more sensitive assays such as ELISAs are used.

Product Details

Target Species
Mouse
Product Form
Purified mouse IgG - liquid
Preparation
Purified IgG prepared from normal mouse serum by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 2 mg
0.09%Sodium Azide
Pack Size: 10 mg
0.09% Sodium Azide (NaN3)
Purity
90-95% by serum protein electrophoresis
Approx. Protein Concentrations
IgG concentration 1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01869
P01865
P03987
P01864
P01868
P01867
P01863
P01844
P01837
P01843
P01845
Entrez Gene
Igk-C
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of IgG

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoassay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Product Specific References

References for IgG

  1. Tanaka, M. et al. (2003) Potential preventive effects of follistatin-related protein/TSC-36 on joint destruction and antagonistic modulation of its autoantibodies in rheumatoid arthritis.
    Int Immunol. 15 (1): 71-7.
  2. Pirotte, S. et al. (2011) Dentin matrix protein 1 induces membrane expression of VE-cadherin on endothelial cells and inhibits VEGF-induced angiogenesis by blocking VEGFR-2 phosphorylation.
    Blood. 1172515-26.
  3. Klein, D. et al. (2015) Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B.
    J Neuroinflammation. 12: 49.
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