IgG
Purified Mouse IgG used for injection into rats to monitor antibody deposition in pripheral nerve tissues by immunofluorescence
Image caption:
Antibodies accumulate in peripheral nerves after passive transfer and lead to macrophage increase. (A) Immunohistochemical localization of antibodies after systemic injection of IgG in the femoral quadriceps (q) and saphenous nerve (s) from 6-month-old P0wt JHD−/− (7 days post injection, DPI) and P0het JHD−/− mice (7DPI and 28DPI). Note stronger IgG immunoreactivity in the endoneurium from P0het JHD−/− mice that received consecutive IgG injections over 28 days (28DPI). Scale bar, 20 μm. (B) Quantification of F4/80-positive profiles in non-injected control and reconstituted mice showing an increase of macrophage numbers in femoral quadriceps nerve from P0het JHD−/− mutants after IgG reconstitution (n = 3 to 5). One-way ANOVA with Bonferroni-Holm correction. *P < 0.05. (C) Quantification of foamy macrophages reveals an increase of phagocytosing macrophages in femoral quadriceps nerves from P0het JHD−/− mice after IgG reconstitution. Note also the presence of phagocytosing macrophages in control-reconstituted animals (+KLH, +IgG Fc) (n = 3 to 5). Kruskal-Wallis test with Bonferroni-Holm correction. *P < 0.05. n.d. (not detected).
From: Klein, D., Groh, J., Weishaupt, A., & Martini, R. (2015). Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B.
Journal of Neuroinflammation, 12, 49.
Secreted by mature B cells, IgG are the most abundant immunoglobulin isotype and are major components of the adaptive immune system. IgG are found in all body fluids where they recognize and bind antigenic determinants exposed at the surface of invading pathogens to neutralize pathogens and activate other defense cells and the complement system.
This Mouse IgG preparation has been prepared from normal mouse serum and purified by ion exchange chromatography, but may contain traces of other immunoglobulin class. By immunoelectrophoresis a single band is seen against anti-Mouse IgG and anti-Mouse serum. Trace amounts of other immunoglobulins present may be detected if more sensitive assays such as ELISAs are used.
Product Details
- Target Species
- Mouse
- Product Form
- Purified mouse IgG - liquid
- Preparation
- Purified IgG prepared from normal mouse serum by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 2 mg
0.09% Sodium Azide Pack Size: 10 mg0.01% Sodium Azide (NaN3) - Purity
- 90-95% by serum protein electrophoresis
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
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- GO Terms
- GO:0003823 antigen binding
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0050766 positive regulation of phagocytosis
- Regulatory
- For research purposes only
Applications of IgG
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunoassay |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG
-
Tanaka, M. et al. (2003) Potential preventive effects of follistatin-related protein/TSC-36 on joint destruction and antagonistic modulation of its autoantibodies in rheumatoid arthritis.
Int Immunol. 15 (1): 71-7. -
Pirotte, S. et al. (2011) Dentin matrix protein 1 induces membrane expression of VE-cadherin on endothelial cells and inhibits VEGF-induced angiogenesis by blocking VEGFR-2 phosphorylation.
Blood. 1172515-26. -
Klein, D. et al. (2015) Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B.
J Neuroinflammation. 12: 49.
