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Purified human Tenascin is a preparation of human tenascin prepared from tissue culture supernatant of the glioblastoma U-251 MG, formally distributed as U-373 MG, an astrocytoid adherent cell line, an over producer of human tenascin. Tenascin is a secreted peptide found in the extracellular matrix that plays an active role in the development of the CNS and mesenchymal derived organs. It is also present in adult tumour vasculature and has functions in cell adhesion.

Target Species
Product Form
Purified protein from tissue culture supernatant - liquid
Purified from tissue culture supernatant of the overproducing cell line, U251, using precipitation, concentration and dialysis.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
>97% by SDS PAGE
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml
Protein Molecular Weight
Migrates at 280-300 kDa in SDS PAGE analysis
For research purposes only
12 months from date of despatch

Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
This product represents a suitable standard for use with 8640-0208 in a direct ELISA system.

References for Tenascin

  1. Maqbool, A. et al. (2016) Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4.
    World J Cardiol. 8 (5): 340-50.

Entrez Gene
GO Terms
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0009611 response to wounding
GO:0045545 syndecan binding


145648 147591 147599 162845 163451 163822 180306A

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