Resistin
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Recombinant Human Resistin
- Product Type
- Recombinant Protein
- Specificity
- Resistin
Antibody Quality and Performance Guarantee
| Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity. Resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2). |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 250 ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified recombinant resistin expressed in E.coli
- Source
- E.coli
- Buffer Solution
- 10mM Sodium Citrate
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- Determined by its ability to stimulate lipolysis in cultured human adipocytes
- Purity
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 19.5 kDa
- Endotoxin Level
- < 1.0 EU/ug
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.2 | 0.4ng/well |
| Functional Assays | |
||
| Western Blotting | |
1.5 | 3.0ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- Adipose TISSUE-SPECIFIC Secretory Factor
- UniProt
- Q9HD89
- Entrez Gene
- RETN
- GO Terms
- GO:0005179 hormone activity
PHP200
157262
162288
164856
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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