Resistin

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Recombinant Human Resistin

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PHP200 E FN WB 25 µg
Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity.

Studies have shown that resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2).

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 250ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant resistin expressed in E.coli.
Buffer Solution
10mM Sodium Citrate
Preservative Stabilisers
None present
Activity
Determined by its ability to stimulate lipolysis in cultured human adipocytes.
Purity
>98% by SDS PAGE and HPLC analysis.
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution
Molecular Weight
19.5kD
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
Q9HD89 Related reagents
Entrez Gene
RETN Related reagents
GO Terms
GO:0005179 hormone activity
Regulatory
For research purposes only

Applications of Resistin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.