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Resistin

Recombinant Human Resistin

Product Type
Recombinant Protein
Specificity
Resistin

Product Code Applications Pack Size List Price Your Price Qty
PHP200
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 25 µg loader
List Price Your Price
loader

Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity.

Resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2).

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 250 ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant resistin expressed in E.coli
Source
E.coli
Buffer Solution
10mM Sodium Citrate
Preservative Stabilisers
None present
Carrier Free
Yes
Activity
Determined by its ability to stimulate lipolysis in cultured human adipocytes
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight
19.5 kDa
Endotoxin Level
< 1.0 EU/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Synonyms
Adipose TISSUE-SPECIFIC Secretory Factor
UniProt
Q9HD89
Entrez Gene
RETN
GO Terms
GO:0005179 hormone activity

PHP200

157262 162288 164856

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