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Prostate Specific Antigen

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Published customer image:
Native Human prostate specific antigen(7820-0504) used for the evaluation of a biosensor performance for the identification and quantification of native PSA in human samples.
Image caption:
Initial test with PSA concentrations in PBS. (a) EIS spectra for: (i) buffer, (ii) 1 pg/ml, (iii) 10 pg/ml, (iv) 1 ng/ml and (v) 10 ng/ml; (b) normalized standard curve for data shown in the Figure 3(a).

From: Ganna Chornokur, Sunil K. Arya, Catherine Phelan, Richard Tanner, and Shekhar Bhansali,
“Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection".
Journal of Sensors, vol. 2011, Article ID 983752.

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Native Human Prostate Specific Antigen

Product Type
Purified Protein
Specificity
Prostate Specific Antigen

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Product Code Applications Pack Size List Price Your Price Qty
7820-0604
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.2 mg loader
List Price Your Price
loader
7820-0504
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader

Native Human prostate specific antigen preparation is a purified form of human prostate-specific antigen (PSA), a glycoprotein of ~30 kDa found mainly in prostatic tissue and seminal fluid. PSA is also present in small quantities in the serum of normal men and is often elevated in the presence of prostate cancer and in other prostatic disorders.

Native Human prostate specific antigen demonstrates high specific activity and low levels of any contaminating proteins, including those with trypsin-like protease activity (Manning et al. 2012).

Target Species
Human
Product Form
Purified protein from human seminal fluid - liquid
Preparation
7820-0604: Sterile filtered through a 0.2 µM membrane
7820-0504: Sterlie filtered through a 0.2 µM membrane
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Purity
7820-0604: >40% by SDS-PAGE
7820-0504: >96% by SDS-PAGE
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
30 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for Prostate Specific Antigen

  1. Chornokur, G. et al. (2011) Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection
    Journal of Sensors, vol. 2011, Article ID 983752
  2. Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection
    Biosensors Journal 1: H110601
  3. Manning, M,L, et al. (2012) Trypsin-like proteolytic contamination of commercially available psa purified from human seminal fluid.
    Prostate. 72 (11): 1233-8.
  4. Manning ML et al. (2013) Proteolysis of complement factors iC3b and C5 by the serine protease prostate-specific antigen in prostatic fluid and seminal plasma.
    J Immunol. 190 (6): 2567-74.
  5. Piletska, E.V. et al. (2014) Microplates with enhanced immobilization capabilities controlled by a magnetic field
    J Chin Adv Mat Soc. 2: 118-29.
  6. Kostova, M.B. et al. (2018) PSA-alpha-2-macroglobulin complex is enzymatically active in the serum of patients with advanced prostate cancer and can degrade circulating peptide hormones.
    Prostate. 78 (11): 819-29.
  7. Li, H. et al. (2020) Comprehensive role of prostate-specific antigen identified with proteomic analysis in prostate cancer.
    J Cell Mol Med. 24 (17): 10202-15.

Functional Assays

Protein Purification

Synonyms
Kallikrein 3
UniProt
P07288
Entrez Gene
KLK3
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0016525 negative regulation of angiogenesis

7820-0604

156089 158185 168279 180628

7820-0504

151339 156849 163307 168691 169381 180308

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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