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Prostate Specific Antigen

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Published customer image:
Native Human prostate specific antigen(7820-0504) used for the evaluation of a biosensor performance for the identification and quantification of native PSA in human samples.
Image caption:
Initial test with PSA concentrations in PBS. (a) EIS spectra for: (i) buffer, (ii) 1 pg/ml, (iii) 10 pg/ml, (iv) 1 ng/ml and (v) 10 ng/ml; (b) normalized standard curve for data shown in the Figure 3(a).

From: Ganna Chornokur, Sunil K. Arya, Catherine Phelan, Richard Tanner, and Shekhar Bhansali,
“Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection".
Journal of Sensors, vol. 2011, Article ID 983752.

Native Human Prostate Specific Antigen

Product Type
Purified Protein
Product Code Applications Pack Size List Price Quantity
7820-0504
Datasheet
E WB
SDS
0.1 mg loader

7820-0604
Datasheet
E
SDS
0.2 mg loader

Native Human prostate specific antigen preparation is a purified form of human prostate-specific antigen (PSA), a glycoprotein of ~30 kDa found mainly in prostatic tissue and seminal fluid. PSA is also present in small quantities in the serum of normal men and is often elevated in the presence of prostate cancer and in other prostatic disorders.

Native Human prostate specific antigen demonstrates high specific activity and low levels of any contaminating proteins, including those with trypsin-like protease activity (Manning et al. 2012).

Product Details

Target Species
Human
Product Form
Purified protein from human seminal fluid - liquid
Preparation
Sterlie filtered through a 0.2 µM membrane
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 0.2 mg
0.09%Sodium Azide (NaN3)
Purity
Pack Size: 0.1 mg
>96% by SDS-PAGE
Pack Size: 0.2 mg
>40% by SDS-PAGE
Approx. Protein Concentrations
1.0 mg/ml
Protein Molecular Weight
30 kDa

Storage Information

Storage
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.2 mg
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P07288
Entrez Gene
KLK3
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0016525 negative regulation of angiogenesis
Regulatory
For research purposes only

Applications of Prostate Specific Antigen

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Application Based External Images

Functional Assays

Protein Purification

Product Specific References

References for Prostate Specific Antigen

  1. Manning, M,L, et al. (2012) Trypsin-like proteolytic contamination of commercially available psa purified from human seminal fluid.
    Prostate. 72 (11): 1233-8.
  2. Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection
    Biosensors Journal 1: H110601
  3. Chornokur, G. et al. (2011) Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection
    Journal of Sensors, vol. 2011, Article ID 983752
  4. Manning ML et al. (2013) Proteolysis of complement factors iC3b and C5 by the serine protease prostate-specific antigen in prostatic fluid and seminal plasma.
    J Immunol. 190 (6): 2567-74.
  5. Piletska, E.V. et al. (2014) Microplates with enhanced immobilization capabilities controlled by a magnetic field
    J Chin Adv Mat Soc. 2: 118-29.
  6. Isaacs, J.T. et al. (2018) SYNTHETIC ENHANCEMENT OF THE T-CELL ARMAMENTARIUM AS AN ANTI-CANCER THERAPY
    US Patent application 20180148480 A1
  7. Li, H. et al. (2020) Comprehensive role of prostate‐specific antigen identified with proteomic analysis in prostate cancer
    J Cell Mol Med. 27 Jul. [Epub ahead of print].

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