Prostate Specific Antigen
Native Human prostate specific antigen(7820-0504) used for the evaluation of a biosensor performance for the identification and quantification of native PSA in human samples.
Image caption:
Initial test with PSA concentrations in PBS. (a) EIS spectra for: (i) buffer, (ii) 1 pg/ml, (iii) 10 pg/ml, (iv) 1 ng/ml and (v) 10 ng/ml; (b) normalized standard curve for data shown in the Figure 3(a).
From: Ganna Chornokur, Sunil K. Arya, Catherine Phelan, Richard Tanner, and Shekhar Bhansali,
“Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection".
Journal of Sensors, vol. 2011, Article ID 983752.
Native Human prostate specific antigen preparation is a purified form of human prostate-specific antigen (PSA), a glycoprotein of ~30 kDa found mainly in prostatic tissue and seminal fluid. PSA is also present in small quantities in the serum of normal men and is often elevated in the presence of prostate cancer and in other prostatic disorders.
Native Human prostate specific antigen demonstrates high specific activity and low levels of any contaminating proteins, including those with trypsin-like protease activity (Manning et al. 2012).
Native Human prostate specific antigen demonstrates high specific activity and low levels of any contaminating proteins, including those with trypsin-like protease activity (Manning et al. 2012).
Product Details
- Target Species
- Human
- Product Form
- Purified protein from human seminal fluid - liquid
- Preparation
- Sterlie filtered through a 0.2 µM membrane
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Purity
- Pack Size: 0.2 mg>40% by SDS-PAGEPack Size: 0.1 mg>96% by SDS-PAGE
- Approx. Protein Concentrations
- 1.0 mg/ml
- Protein Molecular Weight
- 30 kDa
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07288
- Entrez Gene
- KLK3
- GO Terms
- GO:0004252 serine-type endopeptidase activity
- GO:0005576 extracellular region
- GO:0006508 proteolysis
- GO:0016525 negative regulation of angiogenesis
- Regulatory
- For research purposes only
Applications of Prostate Specific Antigen
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Prostate Specific Antigen
-
Manning, M,L, et al. (2012) Trypsin-like proteolytic contamination of commercially available psa purified from human seminal fluid.
Prostate. 72 (11): 1233-8. -
Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection
Biosensors Journal 1: H110601 -
Chornokur, G. et al. (2011) Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection
Journal of Sensors, vol. 2011, Article ID 983752 -
Manning ML et al. (2013) Proteolysis of complement factors iC3b and C5 by the serine protease prostate-specific antigen in prostatic fluid and seminal plasma.
J Immunol. 190 (6): 2567-74. -
Piletska, E.V. et al. (2014) Microplates with enhanced immobilization capabilities controlled by a magnetic field
J Chin Adv Mat Soc. 2: 118-29. -
Isaacs, J.T. et al. (2018) SYNTHETIC ENHANCEMENT OF THE T-CELL ARMAMENTARIUM AS AN ANTI-CANCER THERAPY
US Patent application 20180148480 A1 -
Li, H. et al. (2020) Comprehensive role of prostate‐specific antigen identified with proteomic analysis in prostate cancer
J Cell Mol Med. 27 Jul. [Epub ahead of print]. -
Kostova, M.B. et al. (2018) PSA-alpha-2-macroglobulin complex is enzymatically active in the serum of patients with advanced prostate cancer and can degrade circulating peptide hormones.
Prostate. 78 (11): 819-29.
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