Prolactin

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Recombinant Human Prolactin

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1570 FN 10 µg
Recombinant human prolactin is a non-glycosylated, E. coli-derived recombinant protein corresponding to full-length mature human prolactin

Product Details

Target Species
Human
Product Form
Recombinant protein - lyophilised
Reconstitution
Reconstitute with 100 ul distilled water.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
See Buffer solution.
Activity
The ED50 is < 0.065ng/ml, as determined by the dose-dependant stimulation of the proliferation of rat lymphoma Nb2-11.
Purity
95% as determined by SEC-HPLC and SDS-PAGE
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight
23 kDa

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P01236 Related reagents
Entrez Gene
PRL Related reagents
GO Terms
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005148 prolactin receptor binding
GO:0005179 hormone activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0007565 female pregnancy
GO:0007595 lactation
GO:0008283 cell proliferation
GO:0040014 regulation of multicellular organism growth
GO:0046427 positive regulation of JAK-STAT cascade
Regulatory
For research purposes only

Applications of Prolactin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.