Prolactin

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Recombinant Human Prolactin

Product Type: Recombinant Protein

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
OBT1570	FN			10 µg

Summary

Recombinant human prolactin is a non-glycosylated, E. coli-derived recombinant protein corresponding to full-length mature human prolactin

Product Details

Target Species: Human
Product Form: Recombinant protein - lyophilised
Reconstitution: Reconstitute with 100 ul distilled water.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: See Buffer solution.
Activity: The ED50 is < 0.065ng/ml, as determined by the dose-dependant stimulation of the proliferation of rat lymphoma Nb2-11.
Purity: 95% as determined by SEC-HPLC and SDS-PAGE
Approx. Protein Concentrations: Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight: 23 kDa

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life: 12 months from date of despatch.

More Information

UniProt: P01236 Related reagents
Entrez Gene: PRL Related reagents
GO Terms: GO:0005576 extracellular region; GO:0005829 cytosol; GO:0005148 prolactin receptor binding; GO:0005179 hormone activity; GO:0007166 cell surface receptor linked signaling pathway; GO:0007565 female pregnancy; GO:0007595 lactation; GO:0008283 cell proliferation; GO:0040014 regulation of multicellular organism growth; GO:0046427 positive regulation of JAK-STAT cascade
Regulatory: For research purposes only

Applications of Prolactin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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